TY - JOUR
T1 - A protein synthesis-dependent late phase of cerebellar long-term depression
AU - Linden, David J.
N1 - Funding Information:
Thanks to D. Gurfel who provided skillful technical assistance and to D. Ginty, P. Worley, C. Aizenman, K. Narasimhan, A. Parent, and K. Takahashi for helpful suggestions. This work was supported by Public Health Service grant MH51106, the Klingenstein and McKnight Foundations, the Develbiss Fund, and the National Alliance for Research on Schizophrenia and Depression.
PY - 1996/9
Y1 - 1996/9
N2 - Synthesis of new protein has been shown to be required for establishment of a late phase in hippocampal long-term potentiation. Whether a similar requirement is needed to produce the late phase of long-term depression (LTD) remains to be determined. Application of transcription inhibitors or of the translation inhibitor anisomycin, immediately, but not 30 min after glutamate/depolarization conjunction, attenuated a late phase of cerebellar LTD in culture. LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, which lacks nuclear material, returned to baseline values with a time course paralleling that observed with protein synthesis inhibitors in intact cultured Purkinje neurons. These findings suggest that there is a distinct late phase of cerebellar LTD that is dependent upon postsynaptic protein synthesis.
AB - Synthesis of new protein has been shown to be required for establishment of a late phase in hippocampal long-term potentiation. Whether a similar requirement is needed to produce the late phase of long-term depression (LTD) remains to be determined. Application of transcription inhibitors or of the translation inhibitor anisomycin, immediately, but not 30 min after glutamate/depolarization conjunction, attenuated a late phase of cerebellar LTD in culture. LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, which lacks nuclear material, returned to baseline values with a time course paralleling that observed with protein synthesis inhibitors in intact cultured Purkinje neurons. These findings suggest that there is a distinct late phase of cerebellar LTD that is dependent upon postsynaptic protein synthesis.
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U2 - 10.1016/S0896-6273(00)80180-4
DO - 10.1016/S0896-6273(00)80180-4
M3 - Article
C2 - 8816711
AN - SCOPUS:0030247631
SN - 0896-6273
VL - 17
SP - 483
EP - 490
JO - Neuron
JF - Neuron
IS - 3
ER -