A protein chip approach for high-throughput antigen identification and characterization

Shaohui Hu, Yu Li, Guozhen Liu, Qifeng Song, Li Wang, Yuning Han, Yang Zhang, Yali Song, Xiying Yao, Yong Tao, Haipan Zeng, Huanming Yang, Jian Wang, Heng Zhu, Zhi Nan Chen, Lin Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.

Original languageEnglish (US)
Pages (from-to)2151-2161
Number of pages11
JournalProteomics
Volume7
Issue number13
DOIs
StatePublished - Jun 2007

Keywords

  • Biomarker
  • Liver proteins
  • Monoclonal antibody
  • Protein microarray

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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