A protein chip approach for high-throughput antigen identification and characterization

Shaohui Hu; Yu Li; Guozhen Liu; Qifeng Song; Li Wang; Yuning Han; Yang Zhang; Yali Song; Xiying Yao; Yong Tao; Haipan Zeng; Huanming Yang; Jian Wang; Heng Zhu; Zhi Nan Chen; Lin Wu

doi:10.1002/pmic.200600923

A protein chip approach for high-throughput antigen identification and characterization

Shaohui Hu, Yu Li, Guozhen Liu, Qifeng Song, Li Wang, Yuning Han, Yang Zhang, Yali Song, Xiying Yao, Yong Tao, Haipan Zeng, Huanming Yang, Jian Wang, Heng Zhu, Zhi Nan Chen, Lin Wu

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.

Original language	English (US)
Pages (from-to)	2151-2161
Number of pages	11
Journal	Proteomics
Volume	7
Issue number	13
DOIs	https://doi.org/10.1002/pmic.200600923
State	Published - Jun 2007

Keywords

Biomarker
Liver proteins
Monoclonal antibody
Protein microarray

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1002/pmic.200600923

Cite this

@article{8ce9b5e36cd0467bb8c76ef31fba9566,

title = "A protein chip approach for high-throughput antigen identification and characterization",

abstract = "Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.",

keywords = "Biomarker, Liver proteins, Monoclonal antibody, Protein microarray",

author = "Shaohui Hu and Yu Li and Guozhen Liu and Qifeng Song and Li Wang and Yuning Han and Yang Zhang and Yali Song and Xiying Yao and Yong Tao and Haipan Zeng and Huanming Yang and Jian Wang and Heng Zhu and Chen, {Zhi Nan} and Lin Wu",

year = "2007",

month = jun,

doi = "10.1002/pmic.200600923",

language = "English (US)",

volume = "7",

pages = "2151--2161",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "13",

}

TY - JOUR

T1 - A protein chip approach for high-throughput antigen identification and characterization

AU - Hu, Shaohui

AU - Li, Yu

AU - Liu, Guozhen

AU - Song, Qifeng

AU - Wang, Li

AU - Han, Yuning

AU - Zhang, Yang

AU - Song, Yali

AU - Yao, Xiying

AU - Tao, Yong

AU - Zeng, Haipan

AU - Yang, Huanming

AU - Wang, Jian

AU - Zhu, Heng

AU - Chen, Zhi Nan

AU - Wu, Lin

PY - 2007/6

Y1 - 2007/6

N2 - Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.

AB - Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.

KW - Biomarker

KW - Liver proteins

KW - Monoclonal antibody

KW - Protein microarray

UR - http://www.scopus.com/inward/record.url?scp=34447557663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447557663&partnerID=8YFLogxK

U2 - 10.1002/pmic.200600923

DO - 10.1002/pmic.200600923

M3 - Article

C2 - 17549792

AN - SCOPUS:34447557663

SN - 1615-9853

VL - 7

SP - 2151

EP - 2161

JO - Proteomics

JF - Proteomics

IS - 13

ER -

A protein chip approach for high-throughput antigen identification and characterization

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this