TY - JOUR
T1 - A protective and broadly cross-neutralizing epitope of human papillomavirus L2
AU - Gambhira, Ratish
AU - Karanam, Balasubramanyam
AU - Jagu, Subhashini
AU - Roberts, Jeffrey N.
AU - Buck, Christopher B.
AU - Bossis, Ioannis
AU - Alphs, Hannah
AU - Culp, Timothy
AU - Christensen, Neil D.
AU - Roden, Richard B.S.
PY - 2007/12
Y1 - 2007/12
N2 - We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.
AB - We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.
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U2 - 10.1128/JVI.00936-07
DO - 10.1128/JVI.00936-07
M3 - Article
C2 - 17928339
AN - SCOPUS:37049002453
SN - 0022-538X
VL - 81
SP - 13927
EP - 13931
JO - Journal of virology
JF - Journal of virology
IS - 24
ER -