A proposed method for assessing plasma hypertonicity in vivo

J. D. Stookey, M. Burg, D. E. Sellmeyer, J. E. Greenleaf, A. Arieff, L. Van Hove, C. Gardner, J. C. King

Research output: Contribution to journalArticle

Abstract

Indices of plasma hypertonicity, elevated plasma concentrations of solutes that draw fluid out of cells by osmosis, are needed to pursue hypertonicity as a possible risk factor for obesity and chronic disease. This paper proposes a new index that may be more sensitive to mild hypertonicity in vivo at a point in time than traditional measures. The index compares mean corpuscular volume (MCV) estimates from diluted (in solution by automated cell counter) and nondiluted blood (calculated from manual hematocrit, MCV = Hct/RBC*106). A larger Auto vs Manual MCV (>2fl) in vitro indicates hypertonicity in vivo if the cell counter diluent is isotonic with the threshold for plasma vasopressin (PVP) release and PVP is detectable in plasma (>0.5 pg/ml). To evaluate this principle of concept, hypertonicity was induced by 24-h fluid restriction after a 20 ml/kg water load in four healthy men (20-46 years). Unlike serum and urine indices, the MCV difference-&-PVP index detected hypertonicity in all participants.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalEuropean Journal of Clinical Nutrition
Volume61
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Fingerprint

vasopressin
Erythrocyte Indices
Vasopressins
osmosis
cells
blood serum
methodology
chronic diseases
automobiles
Osmosis
hematocrit
solutes
obesity
erythrocytes
urine
risk factors
Hematocrit
blood
Chronic Disease
Obesity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Stookey, J. D., Burg, M., Sellmeyer, D. E., Greenleaf, J. E., Arieff, A., Van Hove, L., ... King, J. C. (2007). A proposed method for assessing plasma hypertonicity in vivo. European Journal of Clinical Nutrition, 61(1), 143-146. https://doi.org/10.1038/sj.ejcn.1602481

A proposed method for assessing plasma hypertonicity in vivo. / Stookey, J. D.; Burg, M.; Sellmeyer, D. E.; Greenleaf, J. E.; Arieff, A.; Van Hove, L.; Gardner, C.; King, J. C.

In: European Journal of Clinical Nutrition, Vol. 61, No. 1, 01.2007, p. 143-146.

Research output: Contribution to journalArticle

Stookey, JD, Burg, M, Sellmeyer, DE, Greenleaf, JE, Arieff, A, Van Hove, L, Gardner, C & King, JC 2007, 'A proposed method for assessing plasma hypertonicity in vivo', European Journal of Clinical Nutrition, vol. 61, no. 1, pp. 143-146. https://doi.org/10.1038/sj.ejcn.1602481
Stookey JD, Burg M, Sellmeyer DE, Greenleaf JE, Arieff A, Van Hove L et al. A proposed method for assessing plasma hypertonicity in vivo. European Journal of Clinical Nutrition. 2007 Jan;61(1):143-146. https://doi.org/10.1038/sj.ejcn.1602481
Stookey, J. D. ; Burg, M. ; Sellmeyer, D. E. ; Greenleaf, J. E. ; Arieff, A. ; Van Hove, L. ; Gardner, C. ; King, J. C. / A proposed method for assessing plasma hypertonicity in vivo. In: European Journal of Clinical Nutrition. 2007 ; Vol. 61, No. 1. pp. 143-146.
@article{48c04db01db14a338b0db3c738db7fa3,
title = "A proposed method for assessing plasma hypertonicity in vivo",
abstract = "Indices of plasma hypertonicity, elevated plasma concentrations of solutes that draw fluid out of cells by osmosis, are needed to pursue hypertonicity as a possible risk factor for obesity and chronic disease. This paper proposes a new index that may be more sensitive to mild hypertonicity in vivo at a point in time than traditional measures. The index compares mean corpuscular volume (MCV) estimates from diluted (in solution by automated cell counter) and nondiluted blood (calculated from manual hematocrit, MCV = Hct/RBC*106). A larger Auto vs Manual MCV (>2fl) in vitro indicates hypertonicity in vivo if the cell counter diluent is isotonic with the threshold for plasma vasopressin (PVP) release and PVP is detectable in plasma (>0.5 pg/ml). To evaluate this principle of concept, hypertonicity was induced by 24-h fluid restriction after a 20 ml/kg water load in four healthy men (20-46 years). Unlike serum and urine indices, the MCV difference-&-PVP index detected hypertonicity in all participants.",
author = "Stookey, {J. D.} and M. Burg and Sellmeyer, {D. E.} and Greenleaf, {J. E.} and A. Arieff and {Van Hove}, L. and C. Gardner and King, {J. C.}",
year = "2007",
month = "1",
doi = "10.1038/sj.ejcn.1602481",
language = "English (US)",
volume = "61",
pages = "143--146",
journal = "European Journal of Clinical Nutrition",
issn = "0954-3007",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - A proposed method for assessing plasma hypertonicity in vivo

AU - Stookey, J. D.

AU - Burg, M.

AU - Sellmeyer, D. E.

AU - Greenleaf, J. E.

AU - Arieff, A.

AU - Van Hove, L.

AU - Gardner, C.

AU - King, J. C.

PY - 2007/1

Y1 - 2007/1

N2 - Indices of plasma hypertonicity, elevated plasma concentrations of solutes that draw fluid out of cells by osmosis, are needed to pursue hypertonicity as a possible risk factor for obesity and chronic disease. This paper proposes a new index that may be more sensitive to mild hypertonicity in vivo at a point in time than traditional measures. The index compares mean corpuscular volume (MCV) estimates from diluted (in solution by automated cell counter) and nondiluted blood (calculated from manual hematocrit, MCV = Hct/RBC*106). A larger Auto vs Manual MCV (>2fl) in vitro indicates hypertonicity in vivo if the cell counter diluent is isotonic with the threshold for plasma vasopressin (PVP) release and PVP is detectable in plasma (>0.5 pg/ml). To evaluate this principle of concept, hypertonicity was induced by 24-h fluid restriction after a 20 ml/kg water load in four healthy men (20-46 years). Unlike serum and urine indices, the MCV difference-&-PVP index detected hypertonicity in all participants.

AB - Indices of plasma hypertonicity, elevated plasma concentrations of solutes that draw fluid out of cells by osmosis, are needed to pursue hypertonicity as a possible risk factor for obesity and chronic disease. This paper proposes a new index that may be more sensitive to mild hypertonicity in vivo at a point in time than traditional measures. The index compares mean corpuscular volume (MCV) estimates from diluted (in solution by automated cell counter) and nondiluted blood (calculated from manual hematocrit, MCV = Hct/RBC*106). A larger Auto vs Manual MCV (>2fl) in vitro indicates hypertonicity in vivo if the cell counter diluent is isotonic with the threshold for plasma vasopressin (PVP) release and PVP is detectable in plasma (>0.5 pg/ml). To evaluate this principle of concept, hypertonicity was induced by 24-h fluid restriction after a 20 ml/kg water load in four healthy men (20-46 years). Unlike serum and urine indices, the MCV difference-&-PVP index detected hypertonicity in all participants.

UR - http://www.scopus.com/inward/record.url?scp=33845540100&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845540100&partnerID=8YFLogxK

U2 - 10.1038/sj.ejcn.1602481

DO - 10.1038/sj.ejcn.1602481

M3 - Article

C2 - 16855542

AN - SCOPUS:33845540100

VL - 61

SP - 143

EP - 146

JO - European Journal of Clinical Nutrition

JF - European Journal of Clinical Nutrition

SN - 0954-3007

IS - 1

ER -