A proliferative p53-responsive element mediates tumor necrosis factor alpha induction of the human immunodeficiency virus type 1 long terminal repeat

A. Gualberto, M. L. Hixon, T. S. Finco, N. D. Perkins, G. J. Nabel, A. S. Baldwin

Research output: Contribution to journalArticle

Abstract

Transforming mutants of the p53 tumor suppressor gene can positively regulate transcription from several promoters that do not contain known p53 binding sites. Here, we report the identification of a novel p53 binding site in the human immunodeficiency virus long terminal repeat that specifically mediates mutant p53 transactivation. This DNA element was bound by endogenous Jurkat p53 when these cells were stimulated by tumor necrosis factor. Mutation of this sequence inhibited p53 transactivation and tumor necrosis factor inducibility of the human immunodeficiency virus type I long terminal repeat. In addition, this DNA element was found to be sufficient to confer mutant p53 responsiveness on a heterologous minimal promoter. It has been hypothesized that transforming mutants of p53 represent a proliferative conformational stage that can be adopted by the native protein under stimulation by growth factors. The data presented suggest that proliferative and antiproliferative p53 conformations recognize different DNA binding sites in order to mediate distinct biological functions. Thus, transforming mutants of p53 that fold into the proliferative conformation would favor proliferative over antiproliferative functions.

Original languageEnglish (US)
Pages (from-to)3450-3459
Number of pages10
JournalMolecular and Cellular Biology
Volume15
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

HIV Long Terminal Repeat
HIV-1
Tumor Necrosis Factor-alpha
Binding Sites
Transcriptional Activation
DNA
Tumor Suppressor Genes
Intercellular Signaling Peptides and Proteins
Mutation
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Gualberto, A., Hixon, M. L., Finco, T. S., Perkins, N. D., Nabel, G. J., & Baldwin, A. S. (1995). A proliferative p53-responsive element mediates tumor necrosis factor alpha induction of the human immunodeficiency virus type 1 long terminal repeat. Molecular and Cellular Biology, 15(6), 3450-3459.

A proliferative p53-responsive element mediates tumor necrosis factor alpha induction of the human immunodeficiency virus type 1 long terminal repeat. / Gualberto, A.; Hixon, M. L.; Finco, T. S.; Perkins, N. D.; Nabel, G. J.; Baldwin, A. S.

In: Molecular and Cellular Biology, Vol. 15, No. 6, 1995, p. 3450-3459.

Research output: Contribution to journalArticle

Gualberto, A. ; Hixon, M. L. ; Finco, T. S. ; Perkins, N. D. ; Nabel, G. J. ; Baldwin, A. S. / A proliferative p53-responsive element mediates tumor necrosis factor alpha induction of the human immunodeficiency virus type 1 long terminal repeat. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 6. pp. 3450-3459.
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AU - Baldwin, A. S.

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