A prokaryotic membrane anchor sequence: Carboxyl terminus of bacteriophage F1 gene III protein retains it in the membrane

J. D. Boeke, P. Model

Research output: Contribution to journalArticlepeer-review

Abstract

Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydrophobic 'membrane anchor' from another membrane protein, the gene VIII protein, to the truncated gene III protein (by manipulation of the recombinant plasmid) restores membrane anchoring. A model for the relationship of gene III protein with the Escherichia coli membrane is discussed.

Original languageEnglish (US)
Pages (from-to)5200-5204
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number17 I
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • General

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