A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain

Bart De Strooper, Wim Annaert, Philippe Cupers, Paul Saftig, Katleen Craessaerts, Jeffrey Mumm, Eric H. Schroeter, Vincent Schrijvers, Michael S. Wolfe, William J. Ray, Alison Goate, Raphael Kopan

Research output: Contribution to journalArticle

Abstract

Signalling through the receptor protein Notch, which is involved in crucial cell-fate decisions during development, requires ligand-induced cleavage of Notch. This cleavage occurs within the predicted transmembrane domain, releasing the Notch intracellular domain (NICD), and is reminiscent of γ-secretase-mediated cleavage of β-amyloid precursor protein (APP), a critical event in the pathogenesis of Alzheimer's disease. A deficiency in presenilin-1 (PS1) inhibits processing of APP by γ-secretase in mammalian cells, and genetic interactions between Notch and PS1 homologues in Caenorhabditis elegans indicate that the presenilins may modulate the Notch signalling pathway. Here we report that, in mammalian cells, PS1 deficiency also reduces the proteolytic release of NICD from a truncated Notch construct, thus identifying the specific biochemical step of the Notch signalling pathway that is affected by PS1. Moreover, several γ-secretase inhibitors block this same step in Notch processing, indicating that related protease activities are responsible for cleavage within the predicted transmembrane domains of Notch and APP. Thus the targeting of γ-secretase for the treatment of Alzheimer's disease may risk toxicity caused by reduced Notch signalling.

Original languageEnglish (US)
Pages (from-to)518-522
Number of pages5
JournalNature
Volume398
Issue number6727
DOIs
StatePublished - Apr 8 1999
Externally publishedYes

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Presenilin-1
Amyloid Precursor Protein Secretases
Peptide Hydrolases
Notch Receptors
Amyloid beta-Protein Precursor
Alzheimer Disease
Presenilins
Caenorhabditis elegans
Cell Communication
Ligands

ASJC Scopus subject areas

  • General

Cite this

De Strooper, B., Annaert, W., Cupers, P., Saftig, P., Craessaerts, K., Mumm, J., ... Kopan, R. (1999). A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain. Nature, 398(6727), 518-522. https://doi.org/10.1038/19083

A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain. / De Strooper, Bart; Annaert, Wim; Cupers, Philippe; Saftig, Paul; Craessaerts, Katleen; Mumm, Jeffrey; Schroeter, Eric H.; Schrijvers, Vincent; Wolfe, Michael S.; Ray, William J.; Goate, Alison; Kopan, Raphael.

In: Nature, Vol. 398, No. 6727, 08.04.1999, p. 518-522.

Research output: Contribution to journalArticle

De Strooper, B, Annaert, W, Cupers, P, Saftig, P, Craessaerts, K, Mumm, J, Schroeter, EH, Schrijvers, V, Wolfe, MS, Ray, WJ, Goate, A & Kopan, R 1999, 'A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain', Nature, vol. 398, no. 6727, pp. 518-522. https://doi.org/10.1038/19083
De Strooper B, Annaert W, Cupers P, Saftig P, Craessaerts K, Mumm J et al. A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain. Nature. 1999 Apr 8;398(6727):518-522. https://doi.org/10.1038/19083
De Strooper, Bart ; Annaert, Wim ; Cupers, Philippe ; Saftig, Paul ; Craessaerts, Katleen ; Mumm, Jeffrey ; Schroeter, Eric H. ; Schrijvers, Vincent ; Wolfe, Michael S. ; Ray, William J. ; Goate, Alison ; Kopan, Raphael. / A presenilin-1-dependent γ-secretase-like protease mediates release of notch intracellular domain. In: Nature. 1999 ; Vol. 398, No. 6727. pp. 518-522.
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