A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression

Janete M. Cerutti, Rosana Delcelo, Marcelo João Amadei, Claudia Nakabashi, Rui M B Maciel, Bercedis Peterson, Jennifer Shoemaker, Gregory J Riggins

Research output: Contribution to journalArticle

Abstract

Accurate diagnosis of thyroid tumors is challenging. A particular problem is distinguishing between follicular thyroid carcinoma (FTC) and benign follicular thyroid adenoma (FTA), where histology of fine-needle aspirates is not conclusive. It is often necessary to remove healthy thyroid to rule out carcinoma. In order to find markers to improve diagnosis, we quantified gene transcript expression from FTC, FTA, and normal thyroid, revealing 73 differentially expressed transcripts (P ≤ 0.0001). Using an independent set of 23 FTCs, FTAs, and matched normal thyroids, 17 genes with large expression differences were tested by real-time RT-PCR. Four genes (DDIT3, ARG2, ITM1, and C1orf24) differed between the two classes FTC and FTA, and a linear combination of expression levels distinguished FTC from FTA with an estimated predictive accuracy of 0.83. Furthermore, immunohistochemistry for DDIT3 and ARG2 showed consistent staining for carcinoma in an independent set 59 follicular tumors (estimated concordance, 0.76; 95% confidence interval, [0.59, 0.93]). A simple test based on a combination of these markers might improve preoperative diagnosis of thyroid nodules, allowing better treatment decisions and reducing long-term health costs.

Original languageEnglish (US)
Pages (from-to)1234-1242
Number of pages9
JournalJournal of Clinical Investigation
Volume113
Issue number8
DOIs
StatePublished - Apr 2004

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Follicular Adenocarcinoma
Thyroid Neoplasms
Routine Diagnostic Tests
Adenoma
Thyroid Gland
Gene Expression
Carcinoma
Thyroid Nodule
Health Care Costs
Genes
Needles
Real-Time Polymerase Chain Reaction
Neoplasms
Histology
Immunohistochemistry
Confidence Intervals
Staining and Labeling

ASJC Scopus subject areas

  • Medicine(all)

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A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression. / Cerutti, Janete M.; Delcelo, Rosana; Amadei, Marcelo João; Nakabashi, Claudia; Maciel, Rui M B; Peterson, Bercedis; Shoemaker, Jennifer; Riggins, Gregory J.

In: Journal of Clinical Investigation, Vol. 113, No. 8, 04.2004, p. 1234-1242.

Research output: Contribution to journalArticle

Cerutti, JM, Delcelo, R, Amadei, MJ, Nakabashi, C, Maciel, RMB, Peterson, B, Shoemaker, J & Riggins, GJ 2004, 'A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression', Journal of Clinical Investigation, vol. 113, no. 8, pp. 1234-1242. https://doi.org/10.1172/JCI200419617
Cerutti, Janete M. ; Delcelo, Rosana ; Amadei, Marcelo João ; Nakabashi, Claudia ; Maciel, Rui M B ; Peterson, Bercedis ; Shoemaker, Jennifer ; Riggins, Gregory J. / A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression. In: Journal of Clinical Investigation. 2004 ; Vol. 113, No. 8. pp. 1234-1242.
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