A predictive algorithm to identify genes that discriminate individuals with fibromyalgia syndrome diagnosis from healthy controls

Nada Lukkahatai, Brian Walitt, Enrique J. Deandrés-Galiana, Juan Luis Fernández-Martínez, Leorey N. Saligan

Research output: Contribution to journalArticle

Abstract

Objectives: Fibromyalgia syndrome (FMS) is a chronic and often debilitating condition that is characterized by persistent fatigue, pain, bowel abnormalities, and sleep disturbances. Currently, there are no definitive prognostic or diagnostic biomarkers for FMS. This study attempted to utilize a novel predictive algorithm to identify a group of genes whose differential expression discriminated individuals with FMS diagnosis from healthy controls. Methods: Secondary analysis of gene expression data from 28 women with FMS and 19 age-and race-matched healthy women. Expression of discriminatory genes were identified using fold-change differential and Fisher’s ratio (FR). Discriminatory accuracy of the differential expression of these genes was determined using leave-one-out-cross-validation. Functional networks of the discriminating genes were described from the Ingenuity’s Knowledge Base. Results: The small-scale signature contained 57 genes whose expressions were highly discriminatory of the FMS diagnosis. The combination of these high discriminatory genes with FR higher than 1.45 provided a leave-one-out-cross-validation accuracy for the FMS diagnosis of 85.11%. The discriminatory genes were associated with 3 canonical pathways: hepatic stellate cell activation, oxidative phosphorylation, and airway pathology related to COPD. Conclusion: The discriminating genes, especially the 2 with the highest accuracy, are associated with mitochondrial function or oxidative phosphorylation and glutamate signaling. Further validation of the clinical utility of this finding is warranted.

Original languageEnglish (US)
Pages (from-to)2981-2990
Number of pages10
JournalJournal of Pain Research
Volume11
DOIs
StatePublished - Jan 1 2018

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Fibromyalgia
Genes
Gene Expression
Oxidative Phosphorylation
Hepatic Stellate Cells
Knowledge Bases
Gene Regulatory Networks
Chronic Obstructive Pulmonary Disease
Fatigue
Glutamic Acid
Sleep
Biomarkers
Pathology
Pain

Keywords

  • Chronic pain
  • Machine learning
  • Medically unexplained symptoms
  • Microarray

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

A predictive algorithm to identify genes that discriminate individuals with fibromyalgia syndrome diagnosis from healthy controls. / Lukkahatai, Nada; Walitt, Brian; Deandrés-Galiana, Enrique J.; Fernández-Martínez, Juan Luis; Saligan, Leorey N.

In: Journal of Pain Research, Vol. 11, 01.01.2018, p. 2981-2990.

Research output: Contribution to journalArticle

Lukkahatai, Nada ; Walitt, Brian ; Deandrés-Galiana, Enrique J. ; Fernández-Martínez, Juan Luis ; Saligan, Leorey N. / A predictive algorithm to identify genes that discriminate individuals with fibromyalgia syndrome diagnosis from healthy controls. In: Journal of Pain Research. 2018 ; Vol. 11. pp. 2981-2990.
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