TY - JOUR
T1 - A possible role for cold insoluble globulin in chemotactic factor mediated polymorphonuclear leukocyte adherence to plastic
AU - Spagnuolo, Philip J.
AU - Lederman, Michael M.
AU - Schiltz, John
AU - Ellner, Jerrold
AU - Culp, Lloyd
PY - 1982/5/31
Y1 - 1982/5/31
N2 - We examined the potential role of fibronectin in chemotactic factor stimulation of neutrophil adherence to plastic. Monoclonal antibody to human fibronectin significantly reduced chemotactic peptide stimulation of adherence but did not reduce adherence stimulated by phorbol myristate acetate or aggregation stimulated by either agent. Stimulation of neutrophils by chemotactic peptide was also associated with loss of cell surface fibronectin detected by immunofluorescence or binding of radiolabeled collagen. These data suggest that chemotactic peptides stimulate neutrophils to release Fn and that Fn mediates the attachment of neutrophils to plastic surfaces.
AB - We examined the potential role of fibronectin in chemotactic factor stimulation of neutrophil adherence to plastic. Monoclonal antibody to human fibronectin significantly reduced chemotactic peptide stimulation of adherence but did not reduce adherence stimulated by phorbol myristate acetate or aggregation stimulated by either agent. Stimulation of neutrophils by chemotactic peptide was also associated with loss of cell surface fibronectin detected by immunofluorescence or binding of radiolabeled collagen. These data suggest that chemotactic peptides stimulate neutrophils to release Fn and that Fn mediates the attachment of neutrophils to plastic surfaces.
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U2 - 10.1016/0006-291X(82)91134-2
DO - 10.1016/0006-291X(82)91134-2
M3 - Article
C2 - 7049175
AN - SCOPUS:0020371919
SN - 0006-291X
VL - 106
SP - 471
EP - 478
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -