A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink

Research output: Contribution to journalArticlepeer-review

Abstract

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Original languageEnglish (US)
Pages (from-to)345-346
Number of pages2
JournalMGG Molecular & General Genetics
Volume197
Issue number2
DOIs
StatePublished - Nov 1 1984

ASJC Scopus subject areas

  • Genetics

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