A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro

Xinjun He, Gemma A J Kuijpers, Gertrud Goping, Jill A. Kulakusky, Changyu Zheng, Christine Delporte, Chung Ming Tse, Robert S. Redman, Mark Donowitz, Harvey B. Pollard, Bruce J. Baum

Research output: Contribution to journalArticle

Abstract

There are no reported, convenient in vitro models for studying polarized functions in salivary epithelial sells. Accordingly, we examined three often-used salivary cell lines for their ability to form a polarized monolayer on permeable, collagen-coated polycarbonate filters. Only the SMIE line, derived from rat submandibular gland, had this ability. The SMIE cell monolayer exhibited junctional complexes, with a tight-junction-associated protein, ZO-1, localized to cell-cell contact areas. The Na+/K+-ATPase α1-subunit was detected predominantly in the basolateral membranes, while the Na+/H+ exchanger isoform 2 appeared primarily in the apical membranes. Using adenovirus-mediated cDNA transfer, SMIE cells were shown to be capable of routing marker proteins (β-galactosidase ± a nuclear targeting signal, α1-antitrypsin, aquaporin-1) to appropriate locations. Furthermore, this salivary cell monolayer provided a convenient tool for studying aquaporin-1-mediated, osmotically directed, transepithelial fluid movement in vitro. Thus, SMIE cells appear to be a useful experimental model with which to study some polarized functions in a salivary epithelial cell line.

Original languageEnglish (US)
Pages (from-to)375-381
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume435
Issue number3
DOIs
StatePublished - 1998

Fingerprint

Aquaporin 1
Monolayers
polycarbonate
Fluids
Galactosidases
Membranes
Sodium-Hydrogen Antiporter
Adenosine Triphosphatases
Rats
Protein Isoforms
Proteins
Collagen
Complementary DNA
Zonula Occludens-1 Protein
Cells
Cell Line
Submandibular Gland
Adenoviridae
In Vitro Techniques
Theoretical Models

Keywords

  • Aquaporin-1
  • Epithelial cell
  • Facilitated fluid movement
  • Polarity
  • Protein targeting
  • Salivary gland

ASJC Scopus subject areas

  • Physiology

Cite this

He, X., Kuijpers, G. A. J., Goping, G., Kulakusky, J. A., Zheng, C., Delporte, C., ... Baum, B. J. (1998). A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro. Pflugers Archiv European Journal of Physiology, 435(3), 375-381. https://doi.org/10.1007/s004240050526

A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro. / He, Xinjun; Kuijpers, Gemma A J; Goping, Gertrud; Kulakusky, Jill A.; Zheng, Changyu; Delporte, Christine; Tse, Chung Ming; Redman, Robert S.; Donowitz, Mark; Pollard, Harvey B.; Baum, Bruce J.

In: Pflugers Archiv European Journal of Physiology, Vol. 435, No. 3, 1998, p. 375-381.

Research output: Contribution to journalArticle

He, X, Kuijpers, GAJ, Goping, G, Kulakusky, JA, Zheng, C, Delporte, C, Tse, CM, Redman, RS, Donowitz, M, Pollard, HB & Baum, BJ 1998, 'A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro', Pflugers Archiv European Journal of Physiology, vol. 435, no. 3, pp. 375-381. https://doi.org/10.1007/s004240050526
He, Xinjun ; Kuijpers, Gemma A J ; Goping, Gertrud ; Kulakusky, Jill A. ; Zheng, Changyu ; Delporte, Christine ; Tse, Chung Ming ; Redman, Robert S. ; Donowitz, Mark ; Pollard, Harvey B. ; Baum, Bruce J. / A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro. In: Pflugers Archiv European Journal of Physiology. 1998 ; Vol. 435, No. 3. pp. 375-381.
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