A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers

Danijela Jelovac; Julia A. Beaver; Sasidharan Balukrishna; Hong Yuen Wong; Patricia Valda Toro; Ashley Cimino-Mathews; Pedram Argani; Vered Stearns; Lisa Jacobs; Dustin Vandenberg; Jill Kessler; Stacie Jeter; Ben H. Park; Antonio C. Wolff

doi:10.1016/j.humpath.2013.10.016

A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers

Danijela Jelovac, Julia A. Beaver, Sasidharan Balukrishna, Hong Yuen Wong, Patricia Valda Toro, Ashley Cimino-Mathews, Pedram Argani, Vered Stearns, Lisa Jacobs, Dustin Vandenberg, Jill Kessler, Stacie Jeter, Ben H. Park, Antonio C. Wolff

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.

Original language	English (US)
Pages (from-to)	880-883
Number of pages	4
Journal	Human pathology
Volume	45
Issue number	4
DOIs	https://doi.org/10.1016/j.humpath.2013.10.016
State	Published - Apr 2014

Keywords

Breast cancer
Digital PCR
Lung cancer
PIK3CA
Plasma tumor DNA

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/j.humpath.2013.10.016

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Jelovac, D., Beaver, J. A., Balukrishna, S., Wong, H. Y., Toro, P. V., Cimino-Mathews, A., Argani, P., Stearns, V., Jacobs, L., Vandenberg, D., Kessler, J., Jeter, S., Park, B. H., & Wolff, A. C. (2014). A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers. Human pathology, 45(4), 880-883. https://doi.org/10.1016/j.humpath.2013.10.016

Jelovac, D, Beaver, JA, Balukrishna, S, Wong, HY, Toro, PV, Cimino-Mathews, A , Argani, P, Stearns, V, Jacobs, L, Vandenberg, D, Kessler, J, Jeter, S, Park, BH & Wolff, AC 2014, 'A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers', Human pathology, vol. 45, no. 4, pp. 880-883. https://doi.org/10.1016/j.humpath.2013.10.016

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title = "A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers",

abstract = "Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.",

keywords = "Breast cancer, Digital PCR, Lung cancer, PIK3CA, Plasma tumor DNA",

author = "Danijela Jelovac and Beaver, {Julia A.} and Sasidharan Balukrishna and Wong, {Hong Yuen} and Toro, {Patricia Valda} and Ashley Cimino-Mathews and Pedram Argani and Vered Stearns and Lisa Jacobs and Dustin Vandenberg and Jill Kessler and Stacie Jeter and Park, {Ben H.} and Wolff, {Antonio C.}",

note = "Funding Information: This work was supported by the Avon Foundation, the Komen Foundation, the Pearl M. Stetler Fellowship, NIH CA088843 , CA009071 , CA121937 and the Breast Cancer Research Foundation. ",

year = "2014",

month = apr,

doi = "10.1016/j.humpath.2013.10.016",

language = "English (US)",

volume = "45",

pages = "880--883",

journal = "Human pathology",

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AU - Jelovac, Danijela

AU - Beaver, Julia A.

AU - Balukrishna, Sasidharan

AU - Wong, Hong Yuen

AU - Toro, Patricia Valda

AU - Cimino-Mathews, Ashley

AU - Argani, Pedram

AU - Stearns, Vered

AU - Jacobs, Lisa

AU - Vandenberg, Dustin

AU - Kessler, Jill

AU - Jeter, Stacie

AU - Park, Ben H.

AU - Wolff, Antonio C.

N1 - Funding Information: This work was supported by the Avon Foundation, the Komen Foundation, the Pearl M. Stetler Fellowship, NIH CA088843 , CA009071 , CA121937 and the Breast Cancer Research Foundation.

PY - 2014/4

Y1 - 2014/4

N2 - Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.

AB - Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.

KW - Breast cancer

KW - Digital PCR

KW - Lung cancer

KW - PIK3CA

KW - Plasma tumor DNA

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A PIK3CA mutation detected in plasma from a patient with synchronous primary breast and lung cancers

Abstract

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ASJC Scopus subject areas

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