A physical sciences network characterization of non-tumorigenic and metastatic cells

David B. Agus, Jenolyn F. Alexander, Wadih Arap, Shashanka Ashili, Joseph E. Aslan, Robert H. Austin, Vadim Backman, Kelly J. Bethel, Richard Bonneau, Wei Chiang Chen, Chira Chen-Tanyolac, Nathan C. Choi, Steven A. Curley, Matthew Dallas, Dhwanil Damania, Paul C W Davies, Paolo Decuzzi, Laura Dickinson, Luis Estevez-Salmeron, Veronica EstrellaMauro Ferrari, Claudia Fischbach, Jasmine Foo, Stephanie I. Fraley, Christian Frantz, Alexander Fuhrmann, Philippe Gascard, Robert A. Gatenby, Yue Geng, Sharon Gerecht, Robert J. Gillies, Biana Godin, William M. Grady, Alex Greenfield, Courtney Hemphill, Barbara L. Hempstead, Abigail Hielscher, W. Daniel Hillis, Eric C. Holland, Arig Ibrahim-Hashim, Tyler Jacks, Roger H. Johnson, Ahyoung Joo, Jonathan E. Katz, Laimonas Kelbauskas, Carl Kesselman, Michael R. King, K Konstantopoulos, Casey M. Kraning-Rush, Peter Kuhn, Kevin Kung, Brian Kwee, Johnathon N. Lakins, Guillaume Lambert, David Liao, Jonathan D. Licht, Jan T. Liphardt, Liyu Liu, Mark C. Lloyd, Anna Lyubimova, Parag Mallick, John Marko, Owen J T McCarty, Deirdre R. Meldrum, Franziska Michor, Shannon M. Mumenthaler, Vivek Nandakumar, Thomas V. O'Halloran, Steve Oh, Renata Pasqualini, Matthew J. Paszek, Kevin G. Philips, Christopher S. Poultney, Kuldeepsinh Rana, Cynthia A. Reinhart-King, Robert Ros, Gregg L Semenza, Patti Senechal, Michael L. Shuler, Srimeenakshi Srinivasan, Jack R. Staunton, Yolanda Stypula, Hariharan Subramanian, Thea D. Tlsty, Garth W. Tormoen, Yiider Tseng, Alexander Van Oudenaarden, Scott S. Verbridge, Jenny C. Wan, Valerie M. Weaver, Jonathan Widom, Christine Will, Denis Wirtz, Jonathan Wojtkowiak, Pei Hsun Wu

Research output: Contribution to journalArticle

Abstract

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

Original languageEnglish (US)
Article number1449
JournalScientific Reports
Volume3
DOIs
StatePublished - 2013

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Natural Science Disciplines
Neoplasm Metastasis
Cell Line
Mechanics
Proteomics
Neoplasms
Breast
Epithelial Cells
Oxygen
Phenotype

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Agus, D. B., Alexander, J. F., Arap, W., Ashili, S., Aslan, J. E., Austin, R. H., ... Wu, P. H. (2013). A physical sciences network characterization of non-tumorigenic and metastatic cells. Scientific Reports, 3, [1449]. https://doi.org/10.1038/srep01449

A physical sciences network characterization of non-tumorigenic and metastatic cells. / Agus, David B.; Alexander, Jenolyn F.; Arap, Wadih; Ashili, Shashanka; Aslan, Joseph E.; Austin, Robert H.; Backman, Vadim; Bethel, Kelly J.; Bonneau, Richard; Chen, Wei Chiang; Chen-Tanyolac, Chira; Choi, Nathan C.; Curley, Steven A.; Dallas, Matthew; Damania, Dhwanil; Davies, Paul C W; Decuzzi, Paolo; Dickinson, Laura; Estevez-Salmeron, Luis; Estrella, Veronica; Ferrari, Mauro; Fischbach, Claudia; Foo, Jasmine; Fraley, Stephanie I.; Frantz, Christian; Fuhrmann, Alexander; Gascard, Philippe; Gatenby, Robert A.; Geng, Yue; Gerecht, Sharon; Gillies, Robert J.; Godin, Biana; Grady, William M.; Greenfield, Alex; Hemphill, Courtney; Hempstead, Barbara L.; Hielscher, Abigail; Hillis, W. Daniel; Holland, Eric C.; Ibrahim-Hashim, Arig; Jacks, Tyler; Johnson, Roger H.; Joo, Ahyoung; Katz, Jonathan E.; Kelbauskas, Laimonas; Kesselman, Carl; King, Michael R.; Konstantopoulos, K; Kraning-Rush, Casey M.; Kuhn, Peter; Kung, Kevin; Kwee, Brian; Lakins, Johnathon N.; Lambert, Guillaume; Liao, David; Licht, Jonathan D.; Liphardt, Jan T.; Liu, Liyu; Lloyd, Mark C.; Lyubimova, Anna; Mallick, Parag; Marko, John; McCarty, Owen J T; Meldrum, Deirdre R.; Michor, Franziska; Mumenthaler, Shannon M.; Nandakumar, Vivek; O'Halloran, Thomas V.; Oh, Steve; Pasqualini, Renata; Paszek, Matthew J.; Philips, Kevin G.; Poultney, Christopher S.; Rana, Kuldeepsinh; Reinhart-King, Cynthia A.; Ros, Robert; Semenza, Gregg L; Senechal, Patti; Shuler, Michael L.; Srinivasan, Srimeenakshi; Staunton, Jack R.; Stypula, Yolanda; Subramanian, Hariharan; Tlsty, Thea D.; Tormoen, Garth W.; Tseng, Yiider; Van Oudenaarden, Alexander; Verbridge, Scott S.; Wan, Jenny C.; Weaver, Valerie M.; Widom, Jonathan; Will, Christine; Wirtz, Denis; Wojtkowiak, Jonathan; Wu, Pei Hsun.

In: Scientific Reports, Vol. 3, 1449, 2013.

Research output: Contribution to journalArticle

Agus, DB, Alexander, JF, Arap, W, Ashili, S, Aslan, JE, Austin, RH, Backman, V, Bethel, KJ, Bonneau, R, Chen, WC, Chen-Tanyolac, C, Choi, NC, Curley, SA, Dallas, M, Damania, D, Davies, PCW, Decuzzi, P, Dickinson, L, Estevez-Salmeron, L, Estrella, V, Ferrari, M, Fischbach, C, Foo, J, Fraley, SI, Frantz, C, Fuhrmann, A, Gascard, P, Gatenby, RA, Geng, Y, Gerecht, S, Gillies, RJ, Godin, B, Grady, WM, Greenfield, A, Hemphill, C, Hempstead, BL, Hielscher, A, Hillis, WD, Holland, EC, Ibrahim-Hashim, A, Jacks, T, Johnson, RH, Joo, A, Katz, JE, Kelbauskas, L, Kesselman, C, King, MR, Konstantopoulos, K, Kraning-Rush, CM, Kuhn, P, Kung, K, Kwee, B, Lakins, JN, Lambert, G, Liao, D, Licht, JD, Liphardt, JT, Liu, L, Lloyd, MC, Lyubimova, A, Mallick, P, Marko, J, McCarty, OJT, Meldrum, DR, Michor, F, Mumenthaler, SM, Nandakumar, V, O'Halloran, TV, Oh, S, Pasqualini, R, Paszek, MJ, Philips, KG, Poultney, CS, Rana, K, Reinhart-King, CA, Ros, R, Semenza, GL, Senechal, P, Shuler, ML, Srinivasan, S, Staunton, JR, Stypula, Y, Subramanian, H, Tlsty, TD, Tormoen, GW, Tseng, Y, Van Oudenaarden, A, Verbridge, SS, Wan, JC, Weaver, VM, Widom, J, Will, C, Wirtz, D, Wojtkowiak, J & Wu, PH 2013, 'A physical sciences network characterization of non-tumorigenic and metastatic cells', Scientific Reports, vol. 3, 1449. https://doi.org/10.1038/srep01449
Agus, David B. ; Alexander, Jenolyn F. ; Arap, Wadih ; Ashili, Shashanka ; Aslan, Joseph E. ; Austin, Robert H. ; Backman, Vadim ; Bethel, Kelly J. ; Bonneau, Richard ; Chen, Wei Chiang ; Chen-Tanyolac, Chira ; Choi, Nathan C. ; Curley, Steven A. ; Dallas, Matthew ; Damania, Dhwanil ; Davies, Paul C W ; Decuzzi, Paolo ; Dickinson, Laura ; Estevez-Salmeron, Luis ; Estrella, Veronica ; Ferrari, Mauro ; Fischbach, Claudia ; Foo, Jasmine ; Fraley, Stephanie I. ; Frantz, Christian ; Fuhrmann, Alexander ; Gascard, Philippe ; Gatenby, Robert A. ; Geng, Yue ; Gerecht, Sharon ; Gillies, Robert J. ; Godin, Biana ; Grady, William M. ; Greenfield, Alex ; Hemphill, Courtney ; Hempstead, Barbara L. ; Hielscher, Abigail ; Hillis, W. Daniel ; Holland, Eric C. ; Ibrahim-Hashim, Arig ; Jacks, Tyler ; Johnson, Roger H. ; Joo, Ahyoung ; Katz, Jonathan E. ; Kelbauskas, Laimonas ; Kesselman, Carl ; King, Michael R. ; Konstantopoulos, K ; Kraning-Rush, Casey M. ; Kuhn, Peter ; Kung, Kevin ; Kwee, Brian ; Lakins, Johnathon N. ; Lambert, Guillaume ; Liao, David ; Licht, Jonathan D. ; Liphardt, Jan T. ; Liu, Liyu ; Lloyd, Mark C. ; Lyubimova, Anna ; Mallick, Parag ; Marko, John ; McCarty, Owen J T ; Meldrum, Deirdre R. ; Michor, Franziska ; Mumenthaler, Shannon M. ; Nandakumar, Vivek ; O'Halloran, Thomas V. ; Oh, Steve ; Pasqualini, Renata ; Paszek, Matthew J. ; Philips, Kevin G. ; Poultney, Christopher S. ; Rana, Kuldeepsinh ; Reinhart-King, Cynthia A. ; Ros, Robert ; Semenza, Gregg L ; Senechal, Patti ; Shuler, Michael L. ; Srinivasan, Srimeenakshi ; Staunton, Jack R. ; Stypula, Yolanda ; Subramanian, Hariharan ; Tlsty, Thea D. ; Tormoen, Garth W. ; Tseng, Yiider ; Van Oudenaarden, Alexander ; Verbridge, Scott S. ; Wan, Jenny C. ; Weaver, Valerie M. ; Widom, Jonathan ; Will, Christine ; Wirtz, Denis ; Wojtkowiak, Jonathan ; Wu, Pei Hsun. / A physical sciences network characterization of non-tumorigenic and metastatic cells. In: Scientific Reports. 2013 ; Vol. 3.
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abstract = "To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.",
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T1 - A physical sciences network characterization of non-tumorigenic and metastatic cells

AU - Agus, David B.

AU - Alexander, Jenolyn F.

AU - Arap, Wadih

AU - Ashili, Shashanka

AU - Aslan, Joseph E.

AU - Austin, Robert H.

AU - Backman, Vadim

AU - Bethel, Kelly J.

AU - Bonneau, Richard

AU - Chen, Wei Chiang

AU - Chen-Tanyolac, Chira

AU - Choi, Nathan C.

AU - Curley, Steven A.

AU - Dallas, Matthew

AU - Damania, Dhwanil

AU - Davies, Paul C W

AU - Decuzzi, Paolo

AU - Dickinson, Laura

AU - Estevez-Salmeron, Luis

AU - Estrella, Veronica

AU - Ferrari, Mauro

AU - Fischbach, Claudia

AU - Foo, Jasmine

AU - Fraley, Stephanie I.

AU - Frantz, Christian

AU - Fuhrmann, Alexander

AU - Gascard, Philippe

AU - Gatenby, Robert A.

AU - Geng, Yue

AU - Gerecht, Sharon

AU - Gillies, Robert J.

AU - Godin, Biana

AU - Grady, William M.

AU - Greenfield, Alex

AU - Hemphill, Courtney

AU - Hempstead, Barbara L.

AU - Hielscher, Abigail

AU - Hillis, W. Daniel

AU - Holland, Eric C.

AU - Ibrahim-Hashim, Arig

AU - Jacks, Tyler

AU - Johnson, Roger H.

AU - Joo, Ahyoung

AU - Katz, Jonathan E.

AU - Kelbauskas, Laimonas

AU - Kesselman, Carl

AU - King, Michael R.

AU - Konstantopoulos, K

AU - Kraning-Rush, Casey M.

AU - Kuhn, Peter

AU - Kung, Kevin

AU - Kwee, Brian

AU - Lakins, Johnathon N.

AU - Lambert, Guillaume

AU - Liao, David

AU - Licht, Jonathan D.

AU - Liphardt, Jan T.

AU - Liu, Liyu

AU - Lloyd, Mark C.

AU - Lyubimova, Anna

AU - Mallick, Parag

AU - Marko, John

AU - McCarty, Owen J T

AU - Meldrum, Deirdre R.

AU - Michor, Franziska

AU - Mumenthaler, Shannon M.

AU - Nandakumar, Vivek

AU - O'Halloran, Thomas V.

AU - Oh, Steve

AU - Pasqualini, Renata

AU - Paszek, Matthew J.

AU - Philips, Kevin G.

AU - Poultney, Christopher S.

AU - Rana, Kuldeepsinh

AU - Reinhart-King, Cynthia A.

AU - Ros, Robert

AU - Semenza, Gregg L

AU - Senechal, Patti

AU - Shuler, Michael L.

AU - Srinivasan, Srimeenakshi

AU - Staunton, Jack R.

AU - Stypula, Yolanda

AU - Subramanian, Hariharan

AU - Tlsty, Thea D.

AU - Tormoen, Garth W.

AU - Tseng, Yiider

AU - Van Oudenaarden, Alexander

AU - Verbridge, Scott S.

AU - Wan, Jenny C.

AU - Weaver, Valerie M.

AU - Widom, Jonathan

AU - Will, Christine

AU - Wirtz, Denis

AU - Wojtkowiak, Jonathan

AU - Wu, Pei Hsun

PY - 2013

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AB - To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

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