TY - JOUR
T1 - A physical map of the linear unintegrated DNA of visna virus
AU - Clements, J. E.
AU - Narayan, O.
N1 - Funding Information:
The authors gratefully acknowledge the useful suggestions and editorial comments of G. Ketner, the preparation of the manuscript by L. Kelly, and the photographic expertise of S. Burke. This work was funded by a grant from the National Multiple Sclerosis Society.
PY - 1981
Y1 - 1981
N2 - Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 x 106 daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.
AB - Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 x 106 daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.
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M3 - Article
AN - SCOPUS:0019507291
SN - 0166-4328
VL - 3
SP - 412
EP - 415
JO - Behavioural Brain Research
JF - Behavioural Brain Research
IS - 2
ER -