A physical map of the linear unintegrated DNA of visna virus

Janice E. Clements; Opendra Narayan

doi:10.1016/0042-6822(81)90169-0

A physical map of the linear unintegrated DNA of visna virus

Janice E. Clements, Opendra Narayan

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 × 10⁶ daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.

Original language	English (US)
Pages (from-to)	412-415
Number of pages	4
Journal	Virology
Volume	113
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(81)90169-0
State	Published - Aug 1981

ASJC Scopus subject areas

Virology

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title = "A physical map of the linear unintegrated DNA of visna virus",

abstract = "Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 × 106 daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.",

author = "Clements, {Janice E.} and Opendra Narayan",

note = "Funding Information: The authors gratefully acknowledge the useful suggestions and editorial comments of G. Ketner, the preparation of the manuscript by L. Kelly, and the photographic expertise of S. Burke. This work was funded by a grant from the National Multiple Sclerosis Society.",

year = "1981",

month = aug,

doi = "10.1016/0042-6822(81)90169-0",

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T1 - A physical map of the linear unintegrated DNA of visna virus

AU - Clements, Janice E.

AU - Narayan, Opendra

N1 - Funding Information: The authors gratefully acknowledge the useful suggestions and editorial comments of G. Ketner, the preparation of the manuscript by L. Kelly, and the photographic expertise of S. Burke. This work was funded by a grant from the National Multiple Sclerosis Society.

PY - 1981/8

Y1 - 1981/8

N2 - Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 × 106 daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.

AB - Our previous studies have shown that during the lytic infection of sheep cells in culture, visna virus produces an unintegrated viral DNA which is a linear double-stranded molecule with a molecular weight of approximately 6 × 106 daltons. We have now studied this DNA using the method of Southern to locate the cleavage sites of a number of restriction endonucleases on the unintegrated visna viral DNA. Using these sites, we have constructed a physical map of the linear viral DNA. In addition, our analysis has identified two species of closed circular viral DNA in the Hirt supernatant fraction of visna virus-infected cells.

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A physical map of the linear unintegrated DNA of visna virus

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