A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

Nobuhiro Umeda, Tasuku Ueno, Christopher Pohlmeyer, Tetsuo Nagano, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

Abstract

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

Original languageEnglish (US)
Pages (from-to)12-14
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number1
DOIs
StatePublished - Jan 12 2011

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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