A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

Nobuhiro Umeda; Tasuku Ueno; Christopher Pohlmeyer; Tetsuo Nagano; Takanari Inoue

doi:10.1021/ja108258d

A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

Nobuhiro Umeda, Tasuku Ueno, Christopher Pohlmeyer, Tetsuo Nagano, Takanari Inoue

School of Medicine

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

Original language	English (US)
Pages (from-to)	12-14
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	1
DOIs	https://doi.org/10.1021/ja108258d
State	Published - Jan 12 2011

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja108258d

Cite this

@article{1ec1c7900f7949a7aa4c8ae73a673bfd,

title = "A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity",

abstract = "We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.",

author = "Nobuhiro Umeda and Tasuku Ueno and Christopher Pohlmeyer and Tetsuo Nagano and Takanari Inoue",

year = "2011",

month = jan,

day = "12",

doi = "10.1021/ja108258d",

language = "English (US)",

volume = "133",

pages = "12--14",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

AU - Umeda, Nobuhiro

AU - Ueno, Tasuku

AU - Pohlmeyer, Christopher

AU - Nagano, Tetsuo

AU - Inoue, Takanari

PY - 2011/1/12

Y1 - 2011/1/12

N2 - We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

AB - We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

UR - http://www.scopus.com/inward/record.url?scp=78650992316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650992316&partnerID=8YFLogxK

U2 - 10.1021/ja108258d

DO - 10.1021/ja108258d

M3 - Article

C2 - 21142151

AN - SCOPUS:78650992316

SN - 0002-7863

VL - 133

SP - 12

EP - 14

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 1

ER -

A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this