A p53-type response element in the GDF15 promoter confers high specificity for p53 activation

Motonobu Osada, Hannah Lui Park, Min Joo Park, Jun Wei Liu, Guojun Wu, Barry Trink, David Sidransky

Research output: Contribution to journalArticlepeer-review

Abstract

GDF15 is a transcriptional target gene for p53 and its family members, p63 and p73. Its promoter region contains two p53-type response elements, RE1 and RE2, and RE2 confers p53-specific transactivation. RE2 contains several mismatches from the canonical p53 response element (RRRCWWGYYY). Two mismatches in the RRR span and T base of the RE2 core sequence in the most 3′ quarter-site are critical for inhibiting the binding affinity to p63 and p73 and corresponding promoter activity. Our results strongly suggest that differential DNA-binding affinities between p53 family member proteins act, at least in part, to confer specific target gene activation.

Original languageEnglish (US)
Pages (from-to)913-918
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number4
DOIs
StatePublished - Mar 23 2007

Keywords

  • GDF15
  • Gene activation
  • Promoter
  • p53 response element
  • p53 transactivation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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