A nucleoside transporter from Trypanosoma brucei involved in drug resistance

Pascal Mäser, Christine Sütterlin, Anastasia Kralli, Ronald Kaminsky

Research output: Contribution to journalArticle

Abstract

Drug resistance of pathogens is an increasing problem whose underlying mechanisms are not fully understood. Cellular uptake of the major drugs against Trypanosoma brucei spp., the causative agents of sleeping sickness, is thought to occur through an unusual so far unidentified adenosine transporter. Saccharomyces cerevisiae was used in a functional screen to done a gene (TbAT1) from Trypanosoma brucei brucei that encodes a nucleoside transporter. When expressed in yeast, TbAT1 enabled adenosine uptake and conferred susceptibility to melaminophenyl arsenicals. Drug-resistant trypanosomes harbor a defective TbAT1 variant. The molecular identification of the entry route of trypanocides opens the way to approaches for diagnosis and treatment of drug-resistant sleeping sickness.

Original languageEnglish (US)
Pages (from-to)242-244
Number of pages3
JournalScience
Volume285
Issue number5425
DOIs
StatePublished - Jul 9 1999
Externally publishedYes

ASJC Scopus subject areas

  • General

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