A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells

L. M. Scott, C. I. Civin, P. Rorth, A. D. Friedman

Research output: Contribution to journalArticle

Abstract

Members of the CCAAT/enhancer binding protein (C/EBP) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. In these cell lineages, high levels of C/EBPα are found only in mature, nondividing cells. Using Western blotting and immunohistochemical staining, we have determined the temporal order of expression for C/EBPα, C/EBPβ, and C/EBPδ in differentiating myelomonocytic marrow cells. These studies show a unique temporal pattern of C/EBP isoform expression in the myeloid lineage. In particular, C/EBPα expression is very high in proliferative myelomonocytic cells, and diminishes during phenotypic maturation. While we have detected C/EBPα, C/EBPβ, and C/EBPδ in multiple myeloid leukemia cell lines, and C/EBPα in normal myeloid cells and in de novo human myeloid leukemias, we have not detected these C/EBP isoforms in either erythroid or lymphoid cells. Finally, we show that C/EBPα, C/EBPβ, and C/EBPδ protein and messenger RNA levels correlate in maturing granulocytic cells. The formation of tissue-specific combinations of C/EBP homodimers and heterodimers may allow this family of transcription factors to regulate different sets of genes in adipocytes, hepatocytes, and myelomonocytes.

Original languageEnglish (US)
Pages (from-to)1725-1735
Number of pages11
JournalBlood
Volume80
Issue number7
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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