A novel taenia solium protein that resembles troponin T proteins

Holger Mayta, Kathy Hancock, Robert H Gilman, Roxana Zamudio, Janeth P. Castillo, Min Z. Levine, Hector H. Garcia, Armandoe Gonzalez, Victor C W Tsang

Research output: Contribution to journalArticle

Abstract

Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5′ end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalJournal of Parasitology
Volume95
Issue number3
DOIs
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Taenia solium
troponin T
Troponin T
Cysticercosis
cysticercosis
protein
taeniasis
Taeniasis
oncospheres
Proteins
proteins
antigen
antigens
Antigens
DNA libraries
immunoassay
immunoassays
Immunoenzyme Techniques
Gene Library
cDNA libraries

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Mayta, H., Hancock, K., Gilman, R. H., Zamudio, R., Castillo, J. P., Levine, M. Z., ... Tsang, V. C. W. (2009). A novel taenia solium protein that resembles troponin T proteins. Journal of Parasitology, 95(3), 591-597. https://doi.org/10.1645/GE-1767.1

A novel taenia solium protein that resembles troponin T proteins. / Mayta, Holger; Hancock, Kathy; Gilman, Robert H; Zamudio, Roxana; Castillo, Janeth P.; Levine, Min Z.; Garcia, Hector H.; Gonzalez, Armandoe; Tsang, Victor C W.

In: Journal of Parasitology, Vol. 95, No. 3, 06.2009, p. 591-597.

Research output: Contribution to journalArticle

Mayta, H, Hancock, K, Gilman, RH, Zamudio, R, Castillo, JP, Levine, MZ, Garcia, HH, Gonzalez, A & Tsang, VCW 2009, 'A novel taenia solium protein that resembles troponin T proteins', Journal of Parasitology, vol. 95, no. 3, pp. 591-597. https://doi.org/10.1645/GE-1767.1
Mayta H, Hancock K, Gilman RH, Zamudio R, Castillo JP, Levine MZ et al. A novel taenia solium protein that resembles troponin T proteins. Journal of Parasitology. 2009 Jun;95(3):591-597. https://doi.org/10.1645/GE-1767.1
Mayta, Holger ; Hancock, Kathy ; Gilman, Robert H ; Zamudio, Roxana ; Castillo, Janeth P. ; Levine, Min Z. ; Garcia, Hector H. ; Gonzalez, Armandoe ; Tsang, Victor C W. / A novel taenia solium protein that resembles troponin T proteins. In: Journal of Parasitology. 2009 ; Vol. 95, No. 3. pp. 591-597.
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