A novel taenia solium protein that resembles troponin T proteins

Holger Mayta, Kathy Hancock, Robert H. Gilman, Roxana Zamudio, Janeth P. Castillo, Min Z. Levine, Hector H. Garcia, Armando E. Gonzalez, Victor C.W. Tsang

Research output: Contribution to journalArticle

Abstract

Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5′ end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalJournal of Parasitology
Volume95
Issue number3
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

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  • Cite this

    Mayta, H., Hancock, K., Gilman, R. H., Zamudio, R., Castillo, J. P., Levine, M. Z., Garcia, H. H., Gonzalez, A. E., & Tsang, V. C. W. (2009). A novel taenia solium protein that resembles troponin T proteins. Journal of Parasitology, 95(3), 591-597. https://doi.org/10.1645/GE-1767.1