A novel strategy for the generation of T cell lines lacking expression of endogenous α- and/or β-chain T cell receptor genes

N. Glaichenhaus, C. Davis, K. Bornschlegel, J. P. Allison, N. Shastri

Research output: Contribution to journalArticle

Abstract

Mutant T cell lines that do not express the endogenous α- and/or β-chain genes of the TCR were generated from the αβ TCR/CD3+ tumor cell line C6VL with a combination of classical mutagenesis methods and selection of somatic hybrid variants. This novel strategy obviated the need for repeated mutagenesis and screening of a large number of individual clones. The loss of either the α- or the β-chain expression in the mutant cells was associated with the loss of surface TCR/CD3 complex, which could be rescued by the transfection of appropriate exogenous α- and/or β-chain gene constructs. Because these cells express a single TCR molecule on the cell surface, they are useful for the study of the assembly and function of the αβ TCR. This strategy is also generally applicable for the generation of homozygous mutant cell lines lacking other gene products.

Original languageEnglish (US)
Pages (from-to)2095-2101
Number of pages7
JournalJournal of Immunology
Volume146
Issue number7
StatePublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A novel strategy for the generation of T cell lines lacking expression of endogenous α- and/or β-chain T cell receptor genes'. Together they form a unique fingerprint.

  • Cite this