A novel role of the lumican core protein in bacterial lipopolysaccharide- induced innate immune response

Feng Wu, Neeraj Vij, Luke Roberts, S. Lopez-Briones, Sarah Joyce, Shukti Chakravarti

Research output: Contribution to journalArticle

Abstract

Lumican is an extracellular matrix protein modified as a proteoglycan in some tissues. The core protein with leucinerich repeats, characteristic of the leucine-rich-repeat superfamily, binds collagen fibrils and regulates its structure. In addition, we believe that lumican sequestered in the pericellular matrix interacts with cell surface proteins for specific cellular functions. Here we show that bacterial lipopolysaccharide sensing by the Toll-like receptor 4 signaling pathway and innate immune response is regulated by lumican. Primary cultures of lumican-deficient (Lum-/-) macrophages show impaired innate immune response to lipopolysaccharides with lower induction of tumor necrosis factor α (TNFα) and interleukin-6. Macrophage response to other pathogen-associated molecular patterns is not adversely affected by lumican deficiency, suggesting a specific role for the lumican core protein in the Toll-like receptor 4 pathway. An exogenous recombinant lumican core protein increases lipopolysaccharide-mediated TNFα induction and partially rescues innate immune response in Lum-/- macrophages. We further show that the core protein binds lipopolysaccharide. Immunoprecipitation of lumican from peritoneal lavage coprecipitates CD14, a cell surface lipopolysaccharide-binding protein that is involved in its presentation to Toll-like receptor 4. The Lum-/- mice are hypo-responsive to lipopolysaccharide-induced septic shock, with poor induction of proinflammatory cytokines, TNFα, and interleukins 1β and 6 in the serum. Taken together, the data indicates a novel role for lumican in the presentation of bacterial lipopolysaccharide to CD14 and host response to this bacterial endotoxin.

Original languageEnglish (US)
Pages (from-to)26409-26417
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number36
DOIs
StatePublished - Sep 7 2007

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Bacterial Proteins
Innate Immunity
Lipopolysaccharides
Toll-Like Receptor 4
Macrophages
Proteins
Tumor Necrosis Factor-alpha
Interleukin-6
Extracellular Matrix Proteins
Proteoglycans
Interleukin-1
Cell culture
Endotoxins
Leucine
Lumican
Membrane Proteins
Collagen
Peritoneal Lavage
Tissue
Cytokines

ASJC Scopus subject areas

  • Biochemistry

Cite this

A novel role of the lumican core protein in bacterial lipopolysaccharide- induced innate immune response. / Wu, Feng; Vij, Neeraj; Roberts, Luke; Lopez-Briones, S.; Joyce, Sarah; Chakravarti, Shukti.

In: Journal of Biological Chemistry, Vol. 282, No. 36, 07.09.2007, p. 26409-26417.

Research output: Contribution to journalArticle

Wu, F, Vij, N, Roberts, L, Lopez-Briones, S, Joyce, S & Chakravarti, S 2007, 'A novel role of the lumican core protein in bacterial lipopolysaccharide- induced innate immune response', Journal of Biological Chemistry, vol. 282, no. 36, pp. 26409-26417. https://doi.org/10.1074/jbc.M702402200
Wu, Feng ; Vij, Neeraj ; Roberts, Luke ; Lopez-Briones, S. ; Joyce, Sarah ; Chakravarti, Shukti. / A novel role of the lumican core protein in bacterial lipopolysaccharide- induced innate immune response. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 36. pp. 26409-26417.
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