A novel role for aquaporin-5 in enhancing microtubule organization and stability

Venkataramana Sidhaye, Eric Chau, Vasudha Srivastava, Srinivas Sirimalle, Chinmayee Balabhadrapatruni, Neil R. Aggarwal, Franco D'Alessio, Douglas Robinson, Landon Stuart King

Research output: Contribution to journalArticle

Abstract

Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. In addition to regulating transcellular water permeability, AQP5 can regulate paracellular permeability, though the mechanisms by which this occurs have not been determined. Microtubules also regulate paracellular permeability. Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells. Specifically, reduced levels of AQP5 correlated with lower levels of assembled microtubules and decreased paracellular permeability. In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function.

Original languageEnglish (US)
Article numbere38717
JournalPLoS One
Volume7
Issue number6
DOIs
StatePublished - Jun 8 2012

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Aquaporin 5
aquaporins
Microtubules
microtubules
Permeability
permeability
epithelial cells
Epithelial Cells
Water
Aquaporins
water

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sidhaye, V., Chau, E., Srivastava, V., Sirimalle, S., Balabhadrapatruni, C., Aggarwal, N. R., ... King, L. S. (2012). A novel role for aquaporin-5 in enhancing microtubule organization and stability. PLoS One, 7(6), [e38717]. https://doi.org/10.1371/journal.pone.0038717

A novel role for aquaporin-5 in enhancing microtubule organization and stability. / Sidhaye, Venkataramana; Chau, Eric; Srivastava, Vasudha; Sirimalle, Srinivas; Balabhadrapatruni, Chinmayee; Aggarwal, Neil R.; D'Alessio, Franco; Robinson, Douglas; King, Landon Stuart.

In: PLoS One, Vol. 7, No. 6, e38717, 08.06.2012.

Research output: Contribution to journalArticle

Sidhaye V, Chau E, Srivastava V, Sirimalle S, Balabhadrapatruni C, Aggarwal NR et al. A novel role for aquaporin-5 in enhancing microtubule organization and stability. PLoS One. 2012 Jun 8;7(6). e38717. https://doi.org/10.1371/journal.pone.0038717
Sidhaye, Venkataramana ; Chau, Eric ; Srivastava, Vasudha ; Sirimalle, Srinivas ; Balabhadrapatruni, Chinmayee ; Aggarwal, Neil R. ; D'Alessio, Franco ; Robinson, Douglas ; King, Landon Stuart. / A novel role for aquaporin-5 in enhancing microtubule organization and stability. In: PLoS One. 2012 ; Vol. 7, No. 6.
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