A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L. Adams, Parichat Salee, Kim Dionne, Karen Carroll, Nicole Parrish

Research output: Contribution to journalArticlepeer-review

Abstract

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalJournal of Microbiological Methods
Volume119
DOIs
StatePublished - Dec 1 2015

Keywords

  • MALDI-ToF MS
  • Mycobacterium
  • Protein extraction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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