A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L. Adams; Parichat Salee; Kim Dionne; Karen Carroll; Nicole Parrish

doi:10.1016/j.mimet.2015.09.010

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L. Adams, Parichat Salee, Kim Dionne, Karen Carroll, Nicole Parrish

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

Original language	English (US)
Pages (from-to)	1-3
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	119
DOIs	https://doi.org/10.1016/j.mimet.2015.09.010
State	Published - Dec 1 2015

Keywords

MALDI-ToF MS
Mycobacterium
Protein extraction

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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title = "A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS",

abstract = "Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.",

keywords = "MALDI-ToF MS, Mycobacterium, Protein extraction",

author = "Adams, {La'Tonzia L.} and Parichat Salee and Kim Dionne and Karen Carroll and Nicole Parrish",

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doi = "10.1016/j.mimet.2015.09.010",

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AU - Adams, La'Tonzia L.

AU - Salee, Parichat

AU - Dionne, Kim

AU - Carroll, Karen

AU - Parrish, Nicole

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

AB - Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

KW - MALDI-ToF MS

KW - Mycobacterium

KW - Protein extraction

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ER -

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

Abstract

Keywords

ASJC Scopus subject areas

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