A novel population of T-cell receptor αβ-bearing thymocytes which predominantly expresses a single Vβ gene family

B. J. Fowlkes, A. M. Kruisbeek, H. Ton-That, M. A. Weston, J. E. Coligan, R. H. Schwartz, D. M. Pardoll

Research output: Contribution to journalArticle

Abstract

Recent studies have demonstrated that CD3 is expressed on a subset of thymocytes with a CD4-CD8- (double negative) phenotype1,2. At least some of these cells bear the CD3-associated γδ T-cell receptor (TCR γδ)3,4. Here we describe a second subset of double negative thymocytes which expresses CD3-associated αβ receptors (TCR αβ). Surprisingly, these cells express predominantly the products of a single Vβ; gene family (Vβ8). These CD4-CD8-, TCRαβ+ cells appear relatively late in ontogeny (between birth and day 5 of life) and thus are unlikely to be the precursors to the TCR αβ-bearing cells (CD4+CD8- and CD4 -CD8+) already present at birth. They can be selectively expanded in vitro by stimulation with a monoclonal antibody to V β8 (F23.1)5 in the presence of interleukin I (IL-1). We propose that this cell type is a unique T-cell population distinguishable from typical TCR αβ+ T cells by its CD4 -CD8- phenotype and a restricted TCR Vβ repertoire. Analysis of the unique phenotype of these cells suggests that they may represent the normal counterpart of the defective CD4-CD8 - T cells found in the lpr autoimmune mouse.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalNature
Volume329
Issue number6136
DOIs
StatePublished - Jan 1 1987

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    Fowlkes, B. J., Kruisbeek, A. M., Ton-That, H., Weston, M. A., Coligan, J. E., Schwartz, R. H., & Pardoll, D. M. (1987). A novel population of T-cell receptor αβ-bearing thymocytes which predominantly expresses a single Vβ gene family. Nature, 329(6136), 251-254. https://doi.org/10.1038/329251a0