A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule

Marco Vergelli, Hoang Le, Johannes M. Van Noort, Suhayl Dhib-Jalbut, Henry McFarland, Roland Martin

Research output: Contribution to journalArticle

Abstract

CD56 is a member of the neural cell adhesion molecule family expressed on cells of the central nervous system and also on NK cells. Previous studies suggest the involvement of CD56 in effector-to-target cell conjugation mediated by NK cells. It was shown recently that CD56 is also expressed by subpopulations of CD8+ and CD4+ T cells. The present study describes the functional characteristics of CD4+CD56+ T cell lines established from blood of multiple sclerosis patients by stimulation with myelin basic protein (MBP). CD4+CD56+, MBP-specific T cell lines were able to lyse MBP-pulsed target cells in an HLA class II-restricted fashion. At the same time, they mediated MHC-unrestricted lysis of CD56+ target cells such as CD56+ lymphoid or glial tumor cells, but not of the typical NK target, K562. A number of experimental results including separation of CD4+CD56+ T cells into CD56 high and low expressing populations, cold target inhibition, as well as killing of CD56-transfected cells indicate that homotypic CD56 interactions are involved in the MHC-unrestricted lysis. CD56 interactions are not sufficient but are required for effector/target interaction. Our findings raise the possibility that CD4+CD56+ T cells sharing properties of both typical Ag-specific Th0-like T cells and NK cells might be involved in damage of tissues expressing CD56/neural cell adhesion molecule, such as the central nervous system. Thus, we provide evidence for a novel mechanism that could lead to organ-specific autoreactivity.

Original languageEnglish (US)
Pages (from-to)679-688
Number of pages10
JournalJournal of Immunology
Volume157
Issue number2
StatePublished - Jul 15 1996
Externally publishedYes

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Neural Cell Adhesion Molecules
Myelin Sheath
T-Lymphocytes
Myelin Basic Protein
Natural Killer Cells
Population
Central Nervous System
Cell Line
Health Services Needs and Demand
Glioma
Multiple Sclerosis

ASJC Scopus subject areas

  • Immunology

Cite this

Vergelli, M., Le, H., Van Noort, J. M., Dhib-Jalbut, S., McFarland, H., & Martin, R. (1996). A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule. Journal of Immunology, 157(2), 679-688.

A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule. / Vergelli, Marco; Le, Hoang; Van Noort, Johannes M.; Dhib-Jalbut, Suhayl; McFarland, Henry; Martin, Roland.

In: Journal of Immunology, Vol. 157, No. 2, 15.07.1996, p. 679-688.

Research output: Contribution to journalArticle

Vergelli, M, Le, H, Van Noort, JM, Dhib-Jalbut, S, McFarland, H & Martin, R 1996, 'A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule', Journal of Immunology, vol. 157, no. 2, pp. 679-688.
Vergelli M, Le H, Van Noort JM, Dhib-Jalbut S, McFarland H, Martin R. A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule. Journal of Immunology. 1996 Jul 15;157(2):679-688.
Vergelli, Marco ; Le, Hoang ; Van Noort, Johannes M. ; Dhib-Jalbut, Suhayl ; McFarland, Henry ; Martin, Roland. / A novel population of CD4+CD56+ myelin-reactive T cells lyses target cells expressing CD56/neural cell adhesion molecule. In: Journal of Immunology. 1996 ; Vol. 157, No. 2. pp. 679-688.
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