A novel phenotypic method to screen for plasmid-mediated colistin resistance among enterobacteriales

Drew T. Bell, Yehudit Bergman, Abida Q. Kazmi, Shawna Lewis, Pranita D. Tamma, Patricia J. Simner

Research output: Contribution to journalArticle

Abstract

Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test should be further evaluated to confirm the presence of mcr genes.

Original languageEnglish (US)
Article numbere00040-19
JournalJournal of clinical microbiology
Volume57
Issue number5
DOIs
StatePublished - May 2019

Keywords

  • Colistin
  • Mcr
  • Phenotypic method
  • Plasmid-mediated colistin resistance

ASJC Scopus subject areas

  • Microbiology (medical)

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