A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales

Drew T. Bell, Yehudit Bergman, Abida Q. Kazmi, Shawna Lewis, Pranita Tamma, Patricia Simner

Research output: Contribution to journalArticle

Abstract

Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test should be further evaluated to confirm the presence of mcr genes.

Original languageEnglish (US)
JournalJournal of clinical microbiology
Volume57
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Colistin
Plasmids
Edetic Acid
Genes
Zinc
Public Health
Genotype
Sensitivity and Specificity
Enzymes

Keywords

  • colistin
  • mcr
  • phenotypic method
  • plasmid-mediated colistin resistance

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales. / Bell, Drew T.; Bergman, Yehudit; Kazmi, Abida Q.; Lewis, Shawna; Tamma, Pranita; Simner, Patricia.

In: Journal of clinical microbiology, Vol. 57, No. 5, 01.05.2019.

Research output: Contribution to journalArticle

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