A novel PCR assay for quantification of HIV-1 RNA

Liang Shan, S. Alireza Rabi, Gregory M. Laird, Evelyn E. Eisele, Hao Zhang, Joseph B. Margolick, Robert F. Siliciano

Research output: Contribution to journalArticlepeer-review

Abstract

Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.

Original languageEnglish (US)
Pages (from-to)6521-6525
Number of pages5
JournalJournal of virology
Volume87
Issue number11
DOIs
StatePublished - Jun 2013

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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