TY - JOUR
T1 - A novel method to reduce pericardial adhesion
T2 - A combination technique with hyaluronic acid biocompatible membrane
AU - Naito, Yuji
AU - Shin'oka, Toshiharu
AU - Hibino, Narutoshi
AU - Matsumura, Goki
AU - Kurosawa, Hiromi
PY - 2008/4
Y1 - 2008/4
N2 - Objective: This study was to evaluate the efficacy of the hyarulonic acid (HA) bioabsorbable membrane combined use with both expanded-polytetrafluoroethylene (ePTFE) and autologous pericardium for preventing postoperative pericardial adhesions. Methods: The HA bioresorbable surgical membrane (Seprafilm, Genzyme, Cambridge, Mass) was used with either ePTFE or autologous pericardium in an experimental pericardial adhesion model. Twenty-four beagle dogs were classified as follows; Group A (n = 6): ePTFE only, Group B (n = 6): Seprafilm + ePTFE, Group C (n = 6): autologous pericardium only, Group D (n = 6): Seprafilm + autologous pericardium. Pericardial adhesions were evaluated at necropsy at 4, 8, and 12 weeks. The tenacity of adhesion was graded by macroscopic examination, and the adhesion tissue thickness was analyzed microscopically with an image processing program. The regeneration of mesothelial cells on neo-tissue fibrils were immunohistochemically studied. Results: In groups B and D, the adhesions were significant lower compared with those of control groups in the tenacity (Group A vs B: 2.5 ± 0.55 vs 1.5 ± 0.55, P < 0.05; Group C vs D: 3.2 ± 0.75 vs 0.33 ± 0.52, P < 0.01) and the tissue thickness (Group A vs B: 30.4 ± 12.9 vs 10.3 ± 4.42, P < 0.01; Group C vs D: 22.6 ± 11.5 vs 4.96 ± 4.87, P < 0.01). Immunohistochemically, a single layer of mesothelial cells were regenerated on the surface of neo-tissue fibrils in HA treated groups. Conclusion: The combined use of Seprafilm with either ePTFE or autologous pericardium effectively reduced the formation of pericardial adhesion.
AB - Objective: This study was to evaluate the efficacy of the hyarulonic acid (HA) bioabsorbable membrane combined use with both expanded-polytetrafluoroethylene (ePTFE) and autologous pericardium for preventing postoperative pericardial adhesions. Methods: The HA bioresorbable surgical membrane (Seprafilm, Genzyme, Cambridge, Mass) was used with either ePTFE or autologous pericardium in an experimental pericardial adhesion model. Twenty-four beagle dogs were classified as follows; Group A (n = 6): ePTFE only, Group B (n = 6): Seprafilm + ePTFE, Group C (n = 6): autologous pericardium only, Group D (n = 6): Seprafilm + autologous pericardium. Pericardial adhesions were evaluated at necropsy at 4, 8, and 12 weeks. The tenacity of adhesion was graded by macroscopic examination, and the adhesion tissue thickness was analyzed microscopically with an image processing program. The regeneration of mesothelial cells on neo-tissue fibrils were immunohistochemically studied. Results: In groups B and D, the adhesions were significant lower compared with those of control groups in the tenacity (Group A vs B: 2.5 ± 0.55 vs 1.5 ± 0.55, P < 0.05; Group C vs D: 3.2 ± 0.75 vs 0.33 ± 0.52, P < 0.01) and the tissue thickness (Group A vs B: 30.4 ± 12.9 vs 10.3 ± 4.42, P < 0.01; Group C vs D: 22.6 ± 11.5 vs 4.96 ± 4.87, P < 0.01). Immunohistochemically, a single layer of mesothelial cells were regenerated on the surface of neo-tissue fibrils in HA treated groups. Conclusion: The combined use of Seprafilm with either ePTFE or autologous pericardium effectively reduced the formation of pericardial adhesion.
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U2 - 10.1016/j.jtcvs.2007.10.062
DO - 10.1016/j.jtcvs.2007.10.062
M3 - Article
C2 - 18374766
AN - SCOPUS:40949129887
SN - 0022-5223
VL - 135
SP - 850
EP - 856
JO - Journal of Thoracic and Cardiovascular Surgery
JF - Journal of Thoracic and Cardiovascular Surgery
IS - 4
ER -