A novel mechanism for myocardial stunning involving impaired Ca2+ handling

Song Jung Kim, Raymond K. Kudej, Atsuko Yatani, Young Kwon Kim, Gen Takagi, Ritsu Honda, David A. Colantonio, Jennifer E. Van Eyk, Dorothy E. Vatner, Randall L. Rasmusson, Stephen F. Vatner

Research output: Contribution to journalArticle

Abstract

The mechanism of myocardial stunning has been studied extensively in rodents and is thought to involve a decrease in Ca2+ responsiveness of the myofilaments, degradation of Troponin I (TnI), and no change in Ca2+ handling. We studied the mechanism of stunning in isolated myocytes from chronically instrumented pigs. Myocytes were isolated from the ischemic (stunned) and nonischemic (normal) regions after 90-minute coronary stenosis followed by 60-minute reperfusion. Baseline myocyte contraction was reduced, P< 0.01, in stunned myocytes (6.3±0.4%) compared with normal myocytes (8.8±0.4%). The time for 70% relaxation was prolonged, P<0.01, in stunned myocytes (131±8 ms) compared with normal myocytes (105±5 ms). The impaired contractile function was associated with decreased Ca2+ transients (stunned, 0.33±0.04 versus normal, 0.49±0.05, P<0.01). Action potential measurements in stunned myocytes demonstrated a decrease in plateau potential without a change in resting membrane potential. These changes were associated with decreased L-type Ca2+-current density (stunned, -4.8±0.4 versus normal, -6.6±0.4 pA/pF, P<0.01). There were no differences in TnI, sarcoplasmic reticulum Ca2+ ATPase (SERCA2a), and phospholamban protein quantities. However, the fraction of phosphorylated phospholamban monomer was reduced in stunned myocardium. In rats, stunned myocytes demonstrated reduced systolic contraction but actually accelerated relaxation and no change in Ca2+ transients. Thus, mechanisms of stunning in the pig are radically different from the widely held concepts derived from studies in rodents and involve impaired Ca2+ handling and dephosphorylation of phospholamban, but not TnI degradation.

Original languageEnglish (US)
Pages (from-to)831-837
Number of pages7
JournalCirculation research
Volume89
Issue number9
DOIs
StatePublished - Oct 26 2001

Keywords

  • Action potential
  • Calcium
  • Ischemia
  • Myocyte
  • Stunning

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Kim, S. J., Kudej, R. K., Yatani, A., Kim, Y. K., Takagi, G., Honda, R., Colantonio, D. A., Van Eyk, J. E., Vatner, D. E., Rasmusson, R. L., & Vatner, S. F. (2001). A novel mechanism for myocardial stunning involving impaired Ca2+ handling. Circulation research, 89(9), 831-837. https://doi.org/10.1161/hh2101.098547