A novel luminescence-based serum bactericidal assay for vibrio cholerae reduces assay variation, is time- And cost-effective, and directly measures continuous titer values

Taylor A. Wahlig, Ben J. Brintz, Melanie Prettyman, Andrew S. Azman, Daniel T. Leung

Research output: Contribution to journalArticlepeer-review

Abstract

Cholera remains a significant public health burden worldwide, and better methods for monitoring cholera incidence would enhance the effectiveness of public health interventions. The serum bactericidal assay (SBA) has been used extensively for Vibrio cholerae vaccine assessments and serosurveillance. Current SBA approaches for V. cholerae rely on colony enumeration or optical density (OD600nm) readings to measure viable bacteria following complement-mediated lysis. These methods provide titer values that are constrained to discrete dilution values and rely on bacterial outgrowth, which is time consuming and prone to variation. Detection of bacterial proteins following complement-mediated lysis presents a faster and potentially less variable alternative approach independent of bacterial outgrowth. Here, we present an SBA that measures luciferase luminescence driven by lysis-released adenylate kinase. This approach is faster and less variable than growth-dependent SBAs and directly measures continuous titer values. This novel SBA method can potentially be applied to other bacteria of interest.

Original languageEnglish (US)
Pages (from-to)622-626
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume105
Issue number3
DOIs
StatePublished - Sep 2021

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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