A novel immunoassay for the quantitation of human C4 gene products

J. M. Moulds, F. C. Arnett, C. G. Giles, Robert G Hamilton

Research output: Contribution to journalArticle

Abstract

Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalComplement and Inflammation
Volume7
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Immunoassay
Immunodiffusion
Genes
Epitopes
Immunoglobulin G
Hot Temperature
Monoclonal Antibodies

Keywords

  • C4 isotypes
  • Complement
  • Immunoassay
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

A novel immunoassay for the quantitation of human C4 gene products. / Moulds, J. M.; Arnett, F. C.; Giles, C. G.; Hamilton, Robert G.

In: Complement and Inflammation, Vol. 7, No. 2, 1990, p. 95-101.

Research output: Contribution to journalArticle

Moulds, JM, Arnett, FC, Giles, CG & Hamilton, RG 1990, 'A novel immunoassay for the quantitation of human C4 gene products', Complement and Inflammation, vol. 7, no. 2, pp. 95-101.
Moulds, J. M. ; Arnett, F. C. ; Giles, C. G. ; Hamilton, Robert G. / A novel immunoassay for the quantitation of human C4 gene products. In: Complement and Inflammation. 1990 ; Vol. 7, No. 2. pp. 95-101.
@article{39742b2ed5544323b645d341394ba35f,
title = "A novel immunoassay for the quantitation of human C4 gene products",
abstract = "Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8{\%}, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.",
keywords = "C4 isotypes, Complement, Immunoassay, Systemic lupus erythematosus",
author = "Moulds, {J. M.} and Arnett, {F. C.} and Giles, {C. G.} and Hamilton, {Robert G}",
year = "1990",
language = "English (US)",
volume = "7",
pages = "95--101",
journal = "Complement and Inflammation",
issn = "1012-8204",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - A novel immunoassay for the quantitation of human C4 gene products

AU - Moulds, J. M.

AU - Arnett, F. C.

AU - Giles, C. G.

AU - Hamilton, Robert G

PY - 1990

Y1 - 1990

N2 - Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

AB - Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

KW - C4 isotypes

KW - Complement

KW - Immunoassay

KW - Systemic lupus erythematosus

UR - http://www.scopus.com/inward/record.url?scp=0025072104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025072104&partnerID=8YFLogxK

M3 - Article

C2 - 2225796

AN - SCOPUS:0025072104

VL - 7

SP - 95

EP - 101

JO - Complement and Inflammation

JF - Complement and Inflammation

SN - 1012-8204

IS - 2

ER -