A novel immunoassay for the quantitation of human C4 gene products

J. M. Moulds; F. C. Arnett; C. G. Giles; R. G. Hamilton

doi:10.1159/000463134

A novel immunoassay for the quantitation of human C4 gene products

J. M. Moulds, F. C. Arnett, C. G. Giles, R. G. Hamilton

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

Original language	English (US)
Pages (from-to)	95-101
Number of pages	7
Journal	Complement and Inflammation
Volume	7
Issue number	2
DOIs	https://doi.org/10.1159/000463134
State	Published - 1990
Externally published	Yes

Keywords

C4 isotypes
Complement
Immunoassay
Systemic lupus erythematosus

ASJC Scopus subject areas

Immunology
Hematology

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10.1159/000463134

Cite this

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title = "A novel immunoassay for the quantitation of human C4 gene products",

abstract = "Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.",

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doi = "10.1159/000463134",

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T1 - A novel immunoassay for the quantitation of human C4 gene products

AU - Moulds, J. M.

AU - Arnett, F. C.

AU - Giles, C. G.

AU - Hamilton, R. G.

PY - 1990

Y1 - 1990

N2 - Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

AB - Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

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KW - Complement

KW - Immunoassay

KW - Systemic lupus erythematosus

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A novel immunoassay for the quantitation of human C4 gene products

Abstract

Keywords

ASJC Scopus subject areas

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