A novel immunoassay for the quantitation of human C4 gene products

J. M. Moulds, F. C. Arnett, C. G. Giles, R. G. Hamilton

Research output: Contribution to journalArticle

Abstract

Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregates IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalComplement and Inflammation
Volume7
Issue number2
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Keywords

  • C4 isotypes
  • Complement
  • Immunoassay
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology
  • Hematology

Fingerprint Dive into the research topics of 'A novel immunoassay for the quantitation of human C4 gene products'. Together they form a unique fingerprint.

  • Cite this