TY - JOUR
T1 - A Novel Group of Phospholipase A2s Preferentially Expressed in Type 2 Helper T Cells
AU - Ho, I. Cheng
AU - Arm, Jonathan P.
AU - Bingham, Clifton O.
AU - Choi, Andrew
AU - Austen, K. Frank
AU - Glimcher, Laurie H.
PY - 2001/1/25
Y1 - 2001/1/25
N2 - We report a novel phospholipase A2 (PLA2), group XII (GXII) PLA2, distinct from other cysteine-rich groups with a catalytic histidine motif, by its 20-kDa size and distribution of the 14 cysteine residues within the protein. Alternative spliced forms with distinct subcellular localization, designated GXII-1 and GXII-2, were identified by reverse transcription-polymerase chain reaction. Importantly, GXII PLA 2s, in particular GXII-2 PLA2, and group V PLA 2, but not group X PLA2, were selectively expressed in murine type 2 helper T (Th2) clones and in vitro differentiated mouse CD4 Th2 cells as compared with type 1 helper T clones and cells. Stimulation with anti-CD3 appreciably up-regulated expression of GXII PLA2s and group V PLA2 by steady state analysis of the Th2 cells as compared with type 1 helper T cells. These results suggest that group XII and group V PLA 2s might participate in helper T cell immune response through release of immediate second signals and generation of downstream eicosanoids.
AB - We report a novel phospholipase A2 (PLA2), group XII (GXII) PLA2, distinct from other cysteine-rich groups with a catalytic histidine motif, by its 20-kDa size and distribution of the 14 cysteine residues within the protein. Alternative spliced forms with distinct subcellular localization, designated GXII-1 and GXII-2, were identified by reverse transcription-polymerase chain reaction. Importantly, GXII PLA 2s, in particular GXII-2 PLA2, and group V PLA 2, but not group X PLA2, were selectively expressed in murine type 2 helper T (Th2) clones and in vitro differentiated mouse CD4 Th2 cells as compared with type 1 helper T clones and cells. Stimulation with anti-CD3 appreciably up-regulated expression of GXII PLA2s and group V PLA2 by steady state analysis of the Th2 cells as compared with type 1 helper T cells. These results suggest that group XII and group V PLA 2s might participate in helper T cell immune response through release of immediate second signals and generation of downstream eicosanoids.
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U2 - 10.1074/jbc.M008837200
DO - 10.1074/jbc.M008837200
M3 - Article
C2 - 11278438
AN - SCOPUS:0035947631
SN - 0021-9258
VL - 276
SP - 18321
EP - 18326
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 21
ER -