A Novel Group of Phospholipase A2s Preferentially Expressed in Type 2 Helper T Cells

I. Cheng Ho, Jonathan P. Arm, Clifton O. Bingham, Andrew Choi, K. Frank Austen, Laurie H. Glimcher

Research output: Contribution to journalArticlepeer-review

Abstract

We report a novel phospholipase A2 (PLA2), group XII (GXII) PLA2, distinct from other cysteine-rich groups with a catalytic histidine motif, by its 20-kDa size and distribution of the 14 cysteine residues within the protein. Alternative spliced forms with distinct subcellular localization, designated GXII-1 and GXII-2, were identified by reverse transcription-polymerase chain reaction. Importantly, GXII PLA 2s, in particular GXII-2 PLA2, and group V PLA 2, but not group X PLA2, were selectively expressed in murine type 2 helper T (Th2) clones and in vitro differentiated mouse CD4 Th2 cells as compared with type 1 helper T clones and cells. Stimulation with anti-CD3 appreciably up-regulated expression of GXII PLA2s and group V PLA2 by steady state analysis of the Th2 cells as compared with type 1 helper T cells. These results suggest that group XII and group V PLA 2s might participate in helper T cell immune response through release of immediate second signals and generation of downstream eicosanoids.

Original languageEnglish (US)
Pages (from-to)18321-18326
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number21
DOIs
StatePublished - Jan 25 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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