A Novel Experimental Animal Model of Adult Chronic Hydrocephalus

Ignacio Jusué-Torres, Lee H. Jeon, Eric W. Sankey, Jennifer Lu, Tito Vivas-Buitrago, Joshua A. Crawford, Mikhail Pletnikov, Jiadi Xu, Ari M Blitz, Daniel Herzka, Barbara Crain, Alicia Hulbert, Hugo Guerrero-Cazares, Oscar Gonzalez-Perez, James P. McAllister, Alfredo Quiñones-Hinojosa, Daniele Rigamonti

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The pathogenesis of adult chronic hydrocephalus is not fully understood, and the temporal relationship between development of the radiological changes and neurological deterioration is unknown. OBJECTIVE: To clarify the progression of radiological-histological changes and subsequent clinical manifestations of adult chronic hydrocephalus. METHODS: Kaolin was injected bilaterally into the subarachnoid space overlying the cranial convexities in 20 adult rats. Magnetic resonance imaging (MRI) was obtained by using an 11.7 T scanner at 14, 60, 90, and 120 days after kaolin injection. Locomotor, gait, and cognitive evaluations were performed independently. Kaolin distribution and the associated inflammatory and fibrotic responses were histologically analyzed. RESULTS: Evans index of ventriculomegaly showed significant progressive growth in ventricular size over all time points examined. The greatest enlargement occurred within the first 2 months. Evans index also correlated with the extent of kaolin distribution by MRI and by pathological examination at all time points. First gait changes occurred at 69 days, anxiety at 80, cognitive impairment at 81, and locomotor difficulties after 120 days. Only locomotor deterioration was associated with Evans index or the radiological evaluation of kaolin extension. Inflammatory/fibrotic response was histologically confirmed over the cranial convexities in all rats, and its extension was associated with ventricular size and with the rate of ventricular enlargement. CONCLUSION: Kaolin injected into the subarachnoid space over the cerebral hemispheres of adult rats produces an inflammatory/fibrotic response leading in a slow-onset communicating hydrocephalus that is initially asymptomatic. Increased ventricular size eventually leads to gait, memory, and locomotor impairment closely resembling the course of human adult chronic hydrocephalus. ABBREVIATION: NPH, normal pressure hydrocephalus.

Original languageEnglish (US)
Pages (from-to)746-756
Number of pages11
JournalNeurosurgery
Volume79
Issue number5
DOIs
StatePublished - Nov 1 2016

Fingerprint

Kaolin
Hydrocephalus
Animal Models
Gait
Subarachnoid Space
Magnetic Resonance Imaging
Normal Pressure Hydrocephalus
Cerebrum
Anxiety
Injections
Growth

Keywords

  • Animal model
  • Diagnosis
  • Kaolin
  • Normal pressure hydrocephalus
  • Pathophysiology

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Jusué-Torres, I., Jeon, L. H., Sankey, E. W., Lu, J., Vivas-Buitrago, T., Crawford, J. A., ... Rigamonti, D. (2016). A Novel Experimental Animal Model of Adult Chronic Hydrocephalus. Neurosurgery, 79(5), 746-756. https://doi.org/10.1227/NEU.0000000000001405

A Novel Experimental Animal Model of Adult Chronic Hydrocephalus. / Jusué-Torres, Ignacio; Jeon, Lee H.; Sankey, Eric W.; Lu, Jennifer; Vivas-Buitrago, Tito; Crawford, Joshua A.; Pletnikov, Mikhail; Xu, Jiadi; Blitz, Ari M; Herzka, Daniel; Crain, Barbara; Hulbert, Alicia; Guerrero-Cazares, Hugo; Gonzalez-Perez, Oscar; McAllister, James P.; Quiñones-Hinojosa, Alfredo; Rigamonti, Daniele.

In: Neurosurgery, Vol. 79, No. 5, 01.11.2016, p. 746-756.

Research output: Contribution to journalArticle

Jusué-Torres, I, Jeon, LH, Sankey, EW, Lu, J, Vivas-Buitrago, T, Crawford, JA, Pletnikov, M, Xu, J, Blitz, AM, Herzka, D, Crain, B, Hulbert, A, Guerrero-Cazares, H, Gonzalez-Perez, O, McAllister, JP, Quiñones-Hinojosa, A & Rigamonti, D 2016, 'A Novel Experimental Animal Model of Adult Chronic Hydrocephalus', Neurosurgery, vol. 79, no. 5, pp. 746-756. https://doi.org/10.1227/NEU.0000000000001405
Jusué-Torres I, Jeon LH, Sankey EW, Lu J, Vivas-Buitrago T, Crawford JA et al. A Novel Experimental Animal Model of Adult Chronic Hydrocephalus. Neurosurgery. 2016 Nov 1;79(5):746-756. https://doi.org/10.1227/NEU.0000000000001405
Jusué-Torres, Ignacio ; Jeon, Lee H. ; Sankey, Eric W. ; Lu, Jennifer ; Vivas-Buitrago, Tito ; Crawford, Joshua A. ; Pletnikov, Mikhail ; Xu, Jiadi ; Blitz, Ari M ; Herzka, Daniel ; Crain, Barbara ; Hulbert, Alicia ; Guerrero-Cazares, Hugo ; Gonzalez-Perez, Oscar ; McAllister, James P. ; Quiñones-Hinojosa, Alfredo ; Rigamonti, Daniele. / A Novel Experimental Animal Model of Adult Chronic Hydrocephalus. In: Neurosurgery. 2016 ; Vol. 79, No. 5. pp. 746-756.
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AU - Crawford, Joshua A.

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