@article{6c11768cfd384b69b22984fa090c6444,
title = "A novel epigenetic modulating agent sensitizes pancreatic cells to a chemotherapy agent",
abstract = "Pancreatic ductal adenocarcinoma (PDAC) is expected to be the second leading cause of cancer mortality by 2030. PDAC remains resistant to the majority of systemic chemotherapies. In this paper, we explore if epigenetic sensitization can improve chemotherapy response in PDAC. Multiple PDAC cell lines were tested with serial concentrations of the epigenetic modulators 5-azacitidine (Aza) and guadecitabine (SGI-110). Guadecitabine was effective at inhibiting the expression of DNA Methyltransferase 1 (DNMT1) and in decreasing cell viability at nanomolar concentrations. We also report that guadecitabine has increased efficacy following a delay period or as we reference, a {\textquoteleft}rest period{\textquoteright}. Sensitization with guadecitabine improved response to the chemotherapeutic agent–Irinotecan- as measured by decreased cell viability and accompanied by an increase in caspase activity. Additional studies are needed to understand the mechanism of action.",
author = "Manjusha Thakar and Yue Hu and Michael Morreale and Lane Lerner and Lin, {Wan Ying} and Rupashree Sen and Yi Cai and Enusha Karunasena and Maya Thakar and Soren Saggi and Harold Keer and Nita Ahuja",
note = "Funding Information: Research reported in this publication was supported by the National Cancer Institute under award number R01CA185357 as well as by Astex Pharmaceuticals (N.A.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The funder provided support in the form of salary for author (H. K.), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the author contributions section. Publisher Copyright: {\textcopyright} 2018 Thakar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2018",
month = jun,
doi = "10.1371/journal.pone.0199130",
language = "English (US)",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",
}