A novel downstream regulatory element cooperates with the silencing machinery to repress EPA1 expression in candida glabrata

Verónica Gallegos-García, Shih Jung Pan, Jacqueline Juárez-Cepeda, Candy Y. Ramírez-Zavaleta, Marcela Briones Martin-del-Campo, Verónica Martínez-Jiménez, Irene Castaño, Brendan Cormack, Alejandro de Las Peñas

Research output: Contribution to journalArticlepeer-review

Abstract

Candida glabrata, an opportunistic fungal pathogen, adheres to mammalian epithelial cells; adherence is mediated primarily by the Epa1 adhesin. EPA1 is a member of a large gene family of ~23 paralogues, which encode putative adhesins. In this study, we address how EPA1 transcription is regulated. Our data show that EPA1 expression is subject to two distinct negative regulatory mechanisms. EPA1 transcription is repressed by subtelomeric silencing: the Sir complex (Sir2-Sir4), Rap1, Rif1, yKu70, and yKu80 are required for full repression. Activation of EPA1 occurs immediately after dilution of stationary phase (SP) cells into fresh media; however, transcription is rapidly repressed again, limiting expression to lag phase, just as the cells exit stationary phase. This repression following lag phase requires a cis-acting regulatory negative element (NE) located in the EPA1 3'-intergenic region and is independent of telomere proximity. Bioinformatic analysis shows that there are 10 copies of the NE-like sequence in the C. glabrata genome associated with other EPA genes as well as non-EPA genes.

Original languageEnglish (US)
Pages (from-to)1285-1297
Number of pages13
JournalGenetics
Volume190
Issue number4
DOIs
StatePublished - Apr 2012

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'A novel downstream regulatory element cooperates with the silencing machinery to repress EPA1 expression in candida glabrata'. Together they form a unique fingerprint.

Cite this