TY - JOUR
T1 - A novel, convenient, and inexpensive approach for deriving ISCN (1985) relative lengths
T2 - Validation by a morphometric study of 100 karyotyped metaphase cells
AU - Mark, G. F.L.
AU - Parmenter, M.
AU - Campbell, W.
AU - Mark, R.
AU - Zolnierz, K.
AU - Dunwoodie, D.
AU - Hann, E.
AU - Airall, E.
AU - Santoro, K.
AU - Mark, Y.
PY - 1993
Y1 - 1993
N2 - The present study stemmed from a need for a rapid means of deriving reproducible chromosome measurements. An internal set of standards can serve as the basis for routine, easy, and reliable morphometric comparisons. In this study, a total of 100 karyotyped metaphases were analyzed using the Nestler Run-Mate, a computerized curvilinear measuring tool. The null hypothesis tested was that there are no significant differences between chromosomal relative-length values obtained via this previously untested approach and those cited in ISCN (1985). The results indicate that this new method is not only feasible and adequate but has advantages over the conventional approach, which requires the use of a projector and screen to measure chromosomes in unkaryotyped metaphase spreads; further, it is less expensive and easier than using computerized digitizing tablets, a conclusion supported by time-and-effort measurements. Immediately obvious applications include routine use in clinical cytogenetics laboratories, as well as for fractional length estimations in fluorescent in situ hybridization studies performed in research laboratories that do not have access to expensive automated instrumentation.
AB - The present study stemmed from a need for a rapid means of deriving reproducible chromosome measurements. An internal set of standards can serve as the basis for routine, easy, and reliable morphometric comparisons. In this study, a total of 100 karyotyped metaphases were analyzed using the Nestler Run-Mate, a computerized curvilinear measuring tool. The null hypothesis tested was that there are no significant differences between chromosomal relative-length values obtained via this previously untested approach and those cited in ISCN (1985). The results indicate that this new method is not only feasible and adequate but has advantages over the conventional approach, which requires the use of a projector and screen to measure chromosomes in unkaryotyped metaphase spreads; further, it is less expensive and easier than using computerized digitizing tablets, a conclusion supported by time-and-effort measurements. Immediately obvious applications include routine use in clinical cytogenetics laboratories, as well as for fractional length estimations in fluorescent in situ hybridization studies performed in research laboratories that do not have access to expensive automated instrumentation.
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U2 - 10.1159/000133435
DO - 10.1159/000133435
M3 - Article
C2 - 8422751
AN - SCOPUS:0027530685
SN - 0301-0171
VL - 62
SP - 13
EP - 18
JO - Cytogenetics and cell genetics
JF - Cytogenetics and cell genetics
IS - 1
ER -