Abstract
When trypomastigotes of T. cruzi emerge from cells of the mammalian host, they contain little or no sialic acids on their surfaces. However, rapidly upon entering the circulation, they express a unique cell surface trans-sialidase activity. This enzyme specifically transfers α(2-3)-linked sialic acid from extrinsic host-derived macromolecules to parasite surface molecules, leading to the assembly of Ssp-3, a trypomastigote-specific epitope. The T. cruzi trans-sialidase does not utilize cytidine 5′ monophospho-N-acetylneuraminic acid as a donor substrate, but readily transfers sialic acid from exogenously supplied α(2-3)-sialyllactose. Monoclonal antibodies that recognize sialic acid residues of Ssp-3 inhibit attachment of trypomastigotes to host cells, suggesting that the unusual trans-sialidase provides Ssp-3 with structural features required for target cell recognition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1117-1125 |
| Number of pages | 9 |
| Journal | Cell |
| Volume | 65 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jun 28 1991 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
A novel cell surface trans-sialidase of trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cells. / Schenkman, Sergio; Jiang, Man Shiow; Hart, Gerald Warren; Nussenzweig, Victor.
In: Cell, Vol. 65, No. 7, 28.06.1991, p. 1117-1125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel cell surface trans-sialidase of trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cells
AU - Schenkman, Sergio
AU - Jiang, Man Shiow
AU - Hart, Gerald Warren
AU - Nussenzweig, Victor
PY - 1991/6/28
Y1 - 1991/6/28
N2 - When trypomastigotes of T. cruzi emerge from cells of the mammalian host, they contain little or no sialic acids on their surfaces. However, rapidly upon entering the circulation, they express a unique cell surface trans-sialidase activity. This enzyme specifically transfers α(2-3)-linked sialic acid from extrinsic host-derived macromolecules to parasite surface molecules, leading to the assembly of Ssp-3, a trypomastigote-specific epitope. The T. cruzi trans-sialidase does not utilize cytidine 5′ monophospho-N-acetylneuraminic acid as a donor substrate, but readily transfers sialic acid from exogenously supplied α(2-3)-sialyllactose. Monoclonal antibodies that recognize sialic acid residues of Ssp-3 inhibit attachment of trypomastigotes to host cells, suggesting that the unusual trans-sialidase provides Ssp-3 with structural features required for target cell recognition.
AB - When trypomastigotes of T. cruzi emerge from cells of the mammalian host, they contain little or no sialic acids on their surfaces. However, rapidly upon entering the circulation, they express a unique cell surface trans-sialidase activity. This enzyme specifically transfers α(2-3)-linked sialic acid from extrinsic host-derived macromolecules to parasite surface molecules, leading to the assembly of Ssp-3, a trypomastigote-specific epitope. The T. cruzi trans-sialidase does not utilize cytidine 5′ monophospho-N-acetylneuraminic acid as a donor substrate, but readily transfers sialic acid from exogenously supplied α(2-3)-sialyllactose. Monoclonal antibodies that recognize sialic acid residues of Ssp-3 inhibit attachment of trypomastigotes to host cells, suggesting that the unusual trans-sialidase provides Ssp-3 with structural features required for target cell recognition.
UR - http://www.scopus.com/inward/record.url?scp=0025769326&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025769326&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(91)90008-M
DO - 10.1016/0092-8674(91)90008-M
M3 - Article
C2 - 1712251
AN - SCOPUS:0025769326
VL - 65
SP - 1117
EP - 1125
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -