A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation

Julia E. Prescott, Rebecca C. Osthus, Linda A Lee, Brian C. Lewis, Hyunsuk Shim, John F. Barrett, Qingbin Guo, Anita L. Hawkins, Constance A. Griffin, Chi V. Dang

Research output: Contribution to journalArticle

Abstract

We have identified a novel c-Myc-responsive gene, named JP01, by representational difference analysis. JP01 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JP01 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JP01 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Ratla fibroblasts overexpressing JPO1 were tested and compared with transformed Ratla-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Ratla transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.

Original languageEnglish (US)
Pages (from-to)48276-48284
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number51
StatePublished - Dec 21 2001

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myc Genes
Genes
Assays
Phenotype
Thymus
Methylcellulose
Fibroblasts
Nuclear Proteins
Thymus Gland
Small Intestine
Bone
Colon
Carcinogenesis
Leukocytes
Spleen
Complementary DNA
Bone Marrow
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Prescott, J. E., Osthus, R. C., Lee, L. A., Lewis, B. C., Shim, H., Barrett, J. F., ... Dang, C. V. (2001). A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation. Journal of Biological Chemistry, 276(51), 48276-48284.

A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation. / Prescott, Julia E.; Osthus, Rebecca C.; Lee, Linda A; Lewis, Brian C.; Shim, Hyunsuk; Barrett, John F.; Guo, Qingbin; Hawkins, Anita L.; Griffin, Constance A.; Dang, Chi V.

In: Journal of Biological Chemistry, Vol. 276, No. 51, 21.12.2001, p. 48276-48284.

Research output: Contribution to journalArticle

Prescott, JE, Osthus, RC, Lee, LA, Lewis, BC, Shim, H, Barrett, JF, Guo, Q, Hawkins, AL, Griffin, CA & Dang, CV 2001, 'A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation', Journal of Biological Chemistry, vol. 276, no. 51, pp. 48276-48284.
Prescott JE, Osthus RC, Lee LA, Lewis BC, Shim H, Barrett JF et al. A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation. Journal of Biological Chemistry. 2001 Dec 21;276(51):48276-48284.
Prescott, Julia E. ; Osthus, Rebecca C. ; Lee, Linda A ; Lewis, Brian C. ; Shim, Hyunsuk ; Barrett, John F. ; Guo, Qingbin ; Hawkins, Anita L. ; Griffin, Constance A. ; Dang, Chi V. / A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 51. pp. 48276-48284.
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