A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation

Julia E. Prescott, Rebecca C. Osthus, Linda A. Lee, Brian C. Lewis, Hyunsuk Shim, John F. Barrett, Qingbin Guo, Anita L. Hawkins, Constance A. Griffin, Chi V. Dang

Research output: Contribution to journalArticlepeer-review

Abstract

We have identified a novel c-Myc-responsive gene, named JP01, by representational difference analysis. JP01 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JP01 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JP01 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Ratla fibroblasts overexpressing JPO1 were tested and compared with transformed Ratla-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Ratla transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.

Original languageEnglish (US)
Pages (from-to)48276-48284
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number51
DOIs
StatePublished - Dec 21 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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