A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles

Nupura S. Bhise, Ron B. Shmueli, Jose Gonzalez, Jordan J. Green

Research output: Contribution to journalArticle

Abstract

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

Original languageEnglish (US)
Pages (from-to)367-373
Number of pages7
JournalSmall
Volume8
Issue number3
DOIs
StatePublished - Feb 6 2012

Keywords

  • gene expression
  • nanoparticle tracking
  • nanoparticles
  • polymers
  • regenerative medicine

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Engineering (miscellaneous)

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