A novel anti-inflammatory effect for high density lipoprotein

Scott J. Cameron, Craig N. Morrell, Clare Bao, Annemarie F. Swaim, Annabelle Rodriguez-Oquendo, Charles J. Lowenstein

Research output: Contribution to journalArticle

Abstract

High density lipoprotein has anti-inflammatory effects in addition to mediating reverse cholesterol transport. While many of the chronic anti-inflammatory effects of high density lipoprotein (HDL) are attributed to changes in cell adhesion molecules, little is known about acute signal transduction events elicited by HDL in endothelial cells. We now show that high density lipoprotein decreases endothelial cell exocytosis, the first step in leukocyte trafficking. ApoA-I, a major apolipoprotein of HDL, mediates inhibition of endothelial cell exocytosis by interacting with endothelial scavenger receptor-BI which triggers an intracellular protective signaling cascade involving protein kinase C (PKC). Other apolipoproteins within the HDL particle have only modest effects upon endothelial exocytosis. Using a human primary culture of endothelial cells and murine apo-AI knockout mice, we show that apo-AI prevents endothelial cell exocytosis which limits leukocyte recruitment. These data suggest that high density lipoprotein may inhibit diseases associated with vascular inflammation in part by blocking endothelial exocytosis.

Original languageEnglish (US)
Article numbere0144372
JournalPLoS One
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

Fingerprint

high density lipoprotein
HDL Lipoproteins
anti-inflammatory activity
exocytosis
Anti-Inflammatory Agents
Endothelial cells
Exocytosis
endothelial cells
Endothelial Cells
Apolipoprotein A-I
apolipoproteins
Apolipoproteins
leukocytes
Leukocytes
Scavenger Receptors
Signal transduction
mice
Cell Adhesion Molecules
protein kinase C
Cell culture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cameron, S. J., Morrell, C. N., Bao, C., Swaim, A. F., Rodriguez-Oquendo, A., & Lowenstein, C. J. (2015). A novel anti-inflammatory effect for high density lipoprotein. PLoS One, 10(12), [e0144372]. https://doi.org/10.1371/journal.pone.0144372

A novel anti-inflammatory effect for high density lipoprotein. / Cameron, Scott J.; Morrell, Craig N.; Bao, Clare; Swaim, Annemarie F.; Rodriguez-Oquendo, Annabelle; Lowenstein, Charles J.

In: PLoS One, Vol. 10, No. 12, e0144372, 01.12.2015.

Research output: Contribution to journalArticle

Cameron, SJ, Morrell, CN, Bao, C, Swaim, AF, Rodriguez-Oquendo, A & Lowenstein, CJ 2015, 'A novel anti-inflammatory effect for high density lipoprotein', PLoS One, vol. 10, no. 12, e0144372. https://doi.org/10.1371/journal.pone.0144372
Cameron, Scott J. ; Morrell, Craig N. ; Bao, Clare ; Swaim, Annemarie F. ; Rodriguez-Oquendo, Annabelle ; Lowenstein, Charles J. / A novel anti-inflammatory effect for high density lipoprotein. In: PLoS One. 2015 ; Vol. 10, No. 12.
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