A nonthoracotomy myocardial infarction model in an ovine using autologous platelets

Tyler Spata, Daniel Bobek, Bryan A. Whitson, Sampath Parthasarathy, Peter J. Mohler, Robert S.D. Higgins, Ahmet Kilic

Research output: Contribution to journalArticle

Abstract

Objective. There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI. Methods. Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic "border zone," and remote) zones. Results. Serial serum troponin levels (g/mL) measured 0.0±0.0 at baseline and peaked at 297.4±58.0 on post-embolization day 1, followed by 153.0±38.8 on day 2 and 76.7±19.8 on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis. Conclusion. We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.

Original languageEnglish (US)
Article number938047
JournalBioMed research international
Volume2013
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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