TY - JOUR
T1 - A nonthoracotomy myocardial infarction model in an ovine using autologous platelets
AU - Spata, Tyler
AU - Bobek, Daniel
AU - Whitson, Bryan A.
AU - Parthasarathy, Sampath
AU - Mohler, Peter J.
AU - Higgins, Robert S.D.
AU - Kilic, Ahmet
PY - 2013
Y1 - 2013
N2 - Objective. There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI. Methods. Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic "border zone," and remote) zones. Results. Serial serum troponin levels (g/mL) measured 0.0±0.0 at baseline and peaked at 297.4±58.0 on post-embolization day 1, followed by 153.0±38.8 on day 2 and 76.7±19.8 on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis. Conclusion. We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.
AB - Objective. There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI. Methods. Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic "border zone," and remote) zones. Results. Serial serum troponin levels (g/mL) measured 0.0±0.0 at baseline and peaked at 297.4±58.0 on post-embolization day 1, followed by 153.0±38.8 on day 2 and 76.7±19.8 on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis. Conclusion. We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.
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U2 - 10.1155/2013/938047
DO - 10.1155/2013/938047
M3 - Article
C2 - 24367790
AN - SCOPUS:84893759715
SN - 2314-6133
VL - 2013
JO - BioMed research international
JF - BioMed research international
M1 - 938047
ER -