A non-invasive method to produce pressure ulcers of varying severity in a spinal cord-injured rat model

A. K. Ahmed, C. R. Goodwin, R. Sarabia-Estrada, F. Lay, A. M. Ansari, Charles Jr Steenbergen, C. Pang, R. Cohen, L. J. Born, A. E. Matsangos, C. Ng, G. P. Marti, N. Abu-Bonsrah, N. A. Phillips, Ian Suk, Daniel Sciubba, John Harmon

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Abstract

Study design:Experimental study.Objectives:The objective of this study was to establish a non-invasive model to produce pressure ulcers of varying severity in animals with spinal cord injury (SCI).Setting:The study was conducted at the Johns Hopkins Hospital in Baltimore, Maryland, USA.Methods:A mid-thoracic (T7–T9) left hemisection was performed on Sprague-Dawley rats. At 7 days post SCI, rats received varying degrees of pressure on the left posterior thigh region. Laser Doppler Flowmetry was used to record blood flow. Animals were killed 12 days after SCI. A cardiac puncture was performed for blood chemistry, and full-thickness tissue was harvested for histology.Results:Doppler blood flow after SCI prior to pressure application was 237.808±16.175 PFUs at day 7. Following pressure application, there was a statistically significant decrease in blood flow in all pressure-applied groups in comparison with controls with a mean perfusion of 118.361±18.223 (P

Original languageEnglish (US)
JournalSpinal Cord
DOIs
Publication statusAccepted/In press - Jun 21 2016

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ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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