A non-genetic route to aneuploidy in human cancers

Matej Krajcovic, Nicole B. Johnson, Qiang Sun, Guillaume Normand, Nicholas Hoover, Evelyn Yao, Andrea Richardson, Randall W. King, Edmund S. Cibas, Stuart J. Schnitt, Joan S. Brugge, Michael Overholtzer

Research output: Contribution to journalArticle

Abstract

Aneuploidy is common in human tumours and is often indicative of aggressive disease. Aneuploidy can result from cytokinesis failure, which produces binucleate cells that generate aneuploid offspring with subsequent divisions. In cancers, disruption of cytokinesis is known to result from genetic perturbations to mitotic pathways or checkpoints. Here we describe a non-genetic mechanism of cytokinesis failure that occurs as a direct result of cell-in-cell formation by entosis. Live cells internalized by entosis, which can persist through the cell cycle of host cells, disrupt formation of the contractile ring during host cell division. As a result, cytokinesis frequently fails, generating binucleate cells that produce aneuploid cell lineages. In human breast tumours, multinucleation is associated with cell-in-cell structures. These data define a previously unknown mechanism of cytokinesis failure and aneuploid cell formation that operates in human cancers.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalNature Cell Biology
Volume13
Issue number3
DOIs
StatePublished - Mar 2011
Externally publishedYes

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Cytokinesis
Aneuploidy
Entosis
Neoplasms
Cell-in-Cell Formation
Cell Lineage
Cell Division
Cell Cycle
Breast Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Krajcovic, M., Johnson, N. B., Sun, Q., Normand, G., Hoover, N., Yao, E., ... Overholtzer, M. (2011). A non-genetic route to aneuploidy in human cancers. Nature Cell Biology, 13(3), 324-330. https://doi.org/10.1038/ncb2174

A non-genetic route to aneuploidy in human cancers. / Krajcovic, Matej; Johnson, Nicole B.; Sun, Qiang; Normand, Guillaume; Hoover, Nicholas; Yao, Evelyn; Richardson, Andrea; King, Randall W.; Cibas, Edmund S.; Schnitt, Stuart J.; Brugge, Joan S.; Overholtzer, Michael.

In: Nature Cell Biology, Vol. 13, No. 3, 03.2011, p. 324-330.

Research output: Contribution to journalArticle

Krajcovic, M, Johnson, NB, Sun, Q, Normand, G, Hoover, N, Yao, E, Richardson, A, King, RW, Cibas, ES, Schnitt, SJ, Brugge, JS & Overholtzer, M 2011, 'A non-genetic route to aneuploidy in human cancers', Nature Cell Biology, vol. 13, no. 3, pp. 324-330. https://doi.org/10.1038/ncb2174
Krajcovic M, Johnson NB, Sun Q, Normand G, Hoover N, Yao E et al. A non-genetic route to aneuploidy in human cancers. Nature Cell Biology. 2011 Mar;13(3):324-330. https://doi.org/10.1038/ncb2174
Krajcovic, Matej ; Johnson, Nicole B. ; Sun, Qiang ; Normand, Guillaume ; Hoover, Nicholas ; Yao, Evelyn ; Richardson, Andrea ; King, Randall W. ; Cibas, Edmund S. ; Schnitt, Stuart J. ; Brugge, Joan S. ; Overholtzer, Michael. / A non-genetic route to aneuploidy in human cancers. In: Nature Cell Biology. 2011 ; Vol. 13, No. 3. pp. 324-330.
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