A new resource for characterizing X-linked genes in Drosophila melanogaster

Systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications

R. Kimberley Cook, Megan E. Deal, Jennifer A Deal, Russell D. Garton, C. Adam Brown, Megan E. Ward, Rachel S. Andrade, Eric P. Spana, Thomas C. Kaufman, Kevin R. Cook

Research output: Contribution to journalArticle

Abstract

Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in a vital X-linked gene cannot survive unless there is a wild-type copy of the gene duplicated elsewhere in the genome. Rescuing the lethality of an X-linked mutation with a duplication allows the mutation to be used experimentally in complementation tests and other genetic crosses and it maps the mutated gene to a defined chromosomal region. Duplications can also be used to screen for dosage-dependent enhancers and suppressors of mutant phenotypes as a way to identify genes involved in the same biological process. We describe an ongoing project in Drosophila melanogaster to generate comprehensive coverage and extensive breakpoint subdivision of the X chromosome with megabase-scale X segments borne on Y chromosomes. The in vivo method involves the creation of X inversions on attached-XY chromosomes by FLP-FRT site-specific recombination technology followed by irradiation to induce large internal X deletions. The resulting chromosomes consist of the X tip, a medial X segment placed near the tip by an inversion, and a full Y. A nested set of medial duplicated segments is derived from each inversion precursor. We have constructed a set of inversions on attached-XY chromosomes that enable us to isolate nested duplicated segments from all X regions. To date, our screens have provided a minimum of 78% X coverage with duplication breakpoints spaced a median of nine genes apart. These duplication chromosomes will be valuable resources for rescuing and mapping X-linked mutations and identifying dosage-dependent modifiers of mutant phenotypes.

Original languageEnglish (US)
Pages (from-to)1095-1109
Number of pages15
JournalGenetics
Volume186
Issue number4
DOIs
StatePublished - Dec 2010
Externally publishedYes

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X-Linked Genes
X Chromosome
Drosophila melanogaster
Mutation
Genes
Chromosomes
Genetic Crosses
Genetic Complementation Test
Chromosome Duplication
Phenotype
Biological Phenomena
Y Chromosome
Genetic Recombination
Genome
Technology

ASJC Scopus subject areas

  • Genetics

Cite this

A new resource for characterizing X-linked genes in Drosophila melanogaster : Systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications. / Cook, R. Kimberley; Deal, Megan E.; Deal, Jennifer A; Garton, Russell D.; Brown, C. Adam; Ward, Megan E.; Andrade, Rachel S.; Spana, Eric P.; Kaufman, Thomas C.; Cook, Kevin R.

In: Genetics, Vol. 186, No. 4, 12.2010, p. 1095-1109.

Research output: Contribution to journalArticle

Cook, R. Kimberley ; Deal, Megan E. ; Deal, Jennifer A ; Garton, Russell D. ; Brown, C. Adam ; Ward, Megan E. ; Andrade, Rachel S. ; Spana, Eric P. ; Kaufman, Thomas C. ; Cook, Kevin R. / A new resource for characterizing X-linked genes in Drosophila melanogaster : Systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications. In: Genetics. 2010 ; Vol. 186, No. 4. pp. 1095-1109.
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