A new resource for characterizing X-linked genes in Drosophila melanogaster: Systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications

R. Kimberley Cook, Megan E. Deal, Jennifer A. Deal, Russell D. Garton, C. Adam Brown, Megan E. Ward, Rachel S. Andrade, Eric P. Spana, Thomas C. Kaufman, Kevin R. Cook

Research output: Contribution to journalArticle

Abstract

Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in a vital X-linked gene cannot survive unless there is a wild-type copy of the gene duplicated elsewhere in the genome. Rescuing the lethality of an X-linked mutation with a duplication allows the mutation to be used experimentally in complementation tests and other genetic crosses and it maps the mutated gene to a defined chromosomal region. Duplications can also be used to screen for dosage-dependent enhancers and suppressors of mutant phenotypes as a way to identify genes involved in the same biological process. We describe an ongoing project in Drosophila melanogaster to generate comprehensive coverage and extensive breakpoint subdivision of the X chromosome with megabase-scale X segments borne on Y chromosomes. The in vivo method involves the creation of X inversions on attached-XY chromosomes by FLP-FRT site-specific recombination technology followed by irradiation to induce large internal X deletions. The resulting chromosomes consist of the X tip, a medial X segment placed near the tip by an inversion, and a full Y. A nested set of medial duplicated segments is derived from each inversion precursor. We have constructed a set of inversions on attached-XY chromosomes that enable us to isolate nested duplicated segments from all X regions. To date, our screens have provided a minimum of 78% X coverage with duplication breakpoints spaced a median of nine genes apart. These duplication chromosomes will be valuable resources for rescuing and mapping X-linked mutations and identifying dosage-dependent modifiers of mutant phenotypes.

Original languageEnglish (US)
Pages (from-to)1095-1109
Number of pages15
JournalGenetics
Volume186
Issue number4
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this

Cook, R. K., Deal, M. E., Deal, J. A., Garton, R. D., Brown, C. A., Ward, M. E., Andrade, R. S., Spana, E. P., Kaufman, T. C., & Cook, K. R. (2010). A new resource for characterizing X-linked genes in Drosophila melanogaster: Systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications. Genetics, 186(4), 1095-1109. https://doi.org/10.1534/genetics.110.123265