A new rat model for investigation of subglottic stenosis

Joo Hyun Woo, Gyu Cheol Han, Il Gyu Kang, Sun Tae Kim, Heung Eog Cha, Dong Young Kim

Research output: Contribution to journalArticle

Abstract

Conclusion: This study attempted to develop a new rat model of subglottic stenosis (SGS), resulting from subglottic mucosal injury administered by electrocauterization. Despite failure of the posterior SGS model, the anterior SGS model was considered feasible. Objective: To investigate the feasibility of using rats as an animal model for SGS. Methods: Thirty-seven female Sprague-Dawley rats were assigned to the control group or to the anterior or posterior subglottic injury group. Electrical cauterization was performed on the anterior or posterior 180°of the subglottic mucosa. Animals were euthanized at 4 weeks after injury. Histologic features of the subglottis were evaluated as regards changes in the subglottic lumen, lamina propria, cartilage, and epithelium after hematoxylin and eosin and Masson trichrome staining. Results: Survival in the anterior injury group was 80% (13/15) until 4 weeks, and results of histologic evaluation showed an increase in thickness of lamina propria with fibrosis and cartilage damage, resulting in luminal narrowing. A high rate of mortality was observed in the posterior injury group.

Original languageEnglish (US)
Pages (from-to)276-280
Number of pages5
JournalActa Oto-Laryngologica
Volume133
Issue number3
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

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Keywords

  • Animal model
  • Electrocauterization
  • Subglottic mucosa
  • Upper airway obstruction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Woo, J. H., Han, G. C., Kang, I. G., Kim, S. T., Cha, H. E., & Kim, D. Y. (2013). A new rat model for investigation of subglottic stenosis. Acta Oto-Laryngologica, 133(3), 276-280. https://doi.org/10.3109/00016489.2012.734930