Purpose. To report on an optical imaging system developed for tomography of the chorio-retinal vasculature called chorio-retinal optical sectioning (CROS). Methods. CROS consisted of projecting a narrow laser line at an angle on the retina and viewing a cross sectional image of the vasculature following injection of a fluorescent dye. On the fluorescence optical section (FOS) image, retinal and choroidal vasculature appeared laterally displaced according to their depth location. A 2 mm X 2 mm fundus area was scanned and 40 FOS images were generated. The FOS images were processed to separate vascular components from various depths and construct tomographic images of the vascular layers. CROS was performed following fluorescein and indocyanine green angiography in baboon eyes with normal areas and laser induced lesions. Results. The 80 micron depth resolution of CROS was adequate to separate the retinal and choroidal vasculature. The tomographic images had superior contrast and showed microvasculature that were not clearly apparent on conventional angiography Conclusion. CROS generates high contrast tomographic angiograms that enhance the visualization of abnormalities and is a promising tool that may improve the diagnosis of chorio-retinal diseases.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience