A New Modified Technique for Heterotopic Femoral Heart Transplantation in Rats

Chad R. Gordon, Martha S. Matthews, Daniel R. Lefebvre, Louise F. Strande, Steven W. Marra, Massimiliano Guglielmi, Steven M. Hollenberg, Charles W. Hewitt

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Abbott developed the first experimental accessory heart transplant rat model in 1964. This intra-abdominal model required a labor-intensive aortic anastomosis. In 1971, Heron modified the operation by using sutureless cervical vessel anastomoses. Rao and Lisitza developed a femoral heart accessory transplant model in 1985. Our goal was to improve this femoral model for the study of cardiac transplantation between both syngeneic and allogeneic rats. Methods: ACI and Lewis rats weighing 150 to 350g were used as donors and recipients (n = 12). The left common carotid and left pulmonary arteries were anastomosed to the femoral artery and vein in an end-to-end fashion, respectively. Improved modifications included the use of hemostatic vessel clips, heparinization of both donor and recipient, a ventricular prolene stay-suture for secure graft placement, and transfemoral echocardiography (TFE). Total operative time averaged 61 ± 12 minutes. Results: Femoral accessory transplanted hearts (FATHs) allowed easier pulse palpation and access for TFE versus previously described cervical and intra-abdominal models. This modification allows precise detection of acute graft rejection (AGR) and is defined as absent ventricular contraction in the presence of anastomostic patency. Conclusions: Our new modified technique for heterotopic femoral heart transplantation in rats is a relatively easily learned and reproduced procedure that allows superior allograft access for palpation and improved echocardiographic assessment. Femoral heterotopic heart transplantation remains an effective model for allograft transplantation study.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalJournal of Surgical Research
Volume139
Issue number2
DOIs
StatePublished - May 15 2007
Externally publishedYes

Keywords

  • cardiac allotransplantation
  • femoral heart transplant model
  • heterotopic heart transplantation
  • rat transplant technique
  • vascularized bone marrow transplantation

ASJC Scopus subject areas

  • Surgery

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