A New Method for In Vitro Detection of Bromodeoxyuridine in Serum: A Proof of Concept in a Songbird Species, the Canary

Jennifer M. Barker, Thierry D. Charlier, Gregory F. Ball, Jacques Balthazart

Research output: Contribution to journalArticle

Abstract

Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly in studies of adult neurogenesis. Although researchers are applying this technique to multiple species in various physiological conditions, the rate of BrdU clearance from the serum remains unknown in most cases. Changes in this clearance rate as a function of the species, sex or endocrine condition could however profoundly affect the interpretation of the results. We describe a rapid, sensitive, but simple bioassay for post-injection detection and quantification of BrdU in serum. This procedure was shown to be suitable for determining the length of time a thymidine analogue remains in the bloodstream of one avian species and seems applicable to any vertebrate provided sufficiently large blood samples can be collected. This technique was used to demonstrate that, in canaries, BrdU injected at a dose of 100 mg/kg is no longer available for incorporation into DNA between 30 and 60 min post-injection, a delay shorter than anticipated based on the available literature. Preliminary data suggest a similar fast clearance in Japanese quail and mice.

Original languageEnglish (US)
Article numbere63692
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 14 2013

Fingerprint

Canaries
Songbirds
canaries
songbirds
Bromodeoxyuridine
blood serum
thymidine
injection
Serum
vertebrates
Thymidine
Injections
Vertebrates
neurogenesis
adulthood
Coturnix
blood flow
Bioassay
Neurogenesis
Pathology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A New Method for In Vitro Detection of Bromodeoxyuridine in Serum : A Proof of Concept in a Songbird Species, the Canary. / Barker, Jennifer M.; Charlier, Thierry D.; Ball, Gregory F.; Balthazart, Jacques.

In: PLoS One, Vol. 8, No. 5, e63692, 14.05.2013.

Research output: Contribution to journalArticle

Barker, Jennifer M. ; Charlier, Thierry D. ; Ball, Gregory F. ; Balthazart, Jacques. / A New Method for In Vitro Detection of Bromodeoxyuridine in Serum : A Proof of Concept in a Songbird Species, the Canary. In: PLoS One. 2013 ; Vol. 8, No. 5.
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